Invitrogen

CPT2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

1 Published Figure

3 References

View all (19) CPT2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CPT2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

CPT2 Antibody (PA5-12217) in ICC/IF

Immunocytochemistry analysis of CPT2 in HepG2 cells. Samples were incubated in CPT2 polyclonal antibody (Product # PA5-12217) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Image Gallery

CPT2 Antibody — Generation of mice with a macrophage-specific loss of mitochondrial fatty acid ß-oxidation. A: mRNA for carnitine palmitoyltransferase (CPT) II (Cpt2) in bone marrow-derived macrophages (BMDM) from control [wild-type (WT)] mice and BMDM from mice with a specific knockout of Cpt2 [CPT2 Mɸ-KO (KO), n = 3]. B: Western blot for Cpt2 in control and CPT2 Mɸ-KO BMDM. C: Western blot of mitochondrial proteins in control and CPT2 Mɸ-KO BMDM in the presence and absence of IL-4 stimulation (n = 2). D: oxidation of [1-14C]oleic acid to 14CO2 in control and CPT2 Mɸ-KO BMDM (n = 6). E: oxidation of [U-14C]glucose to 14CO2 in control and CPT2 Mɸ-KO BMDM (n = 6). F: incorporation of [3H]acetate into lipid in control and CPT2 Mɸ-KO BMDM (n = 6). G: [1,2-3H]2-deoxyglucose uptake in control and CPT2 Mɸ-KO BMDM (n = 6). H: steady-state metabolite concentrations in IL-4- or LPS-IFNγ-stimulated control and CPT2 Mɸ-KO BMDM (n = 5-6). Values are means SE. *P < 0.05, ***P < 0.001 for genotype comparison. #P < 0.05, ###P < 0.001 for treatment comparison. {KO}

Product Details

PA5-12217

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:10-1:50

Immunoelectrophoresis (IE)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 431-462 amino acids from the C-terminal region of human CPT2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Ammonium sulfate precipitation, Size-exclusion - Dialysis

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2292215

Target Information

Carnitine palmitoyltransferase II precursor (CPT2) is a nuclear protein which is transported to the mitochondrial inner membrane. CPT2 together with carnitine palmitoyltransferase I oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet.

The Journal of biological chemistry

Selen ES,Rodriguez S,Cavagnini KS,Kim HB,Na CH,Wolfgang MJ

PA5-12217 was used in Western Blotting to show that Pcx is important for maintaining the proper balance of pyruvate metabolism between oxidative and anaplerotic pathways.

Thu Dec 01 00:00:00 EST 2022

HDAC inhibitors elicit metabolic reprogramming by targeting super-enhancers in glioblastoma models.

The Journal of clinical investigation

Nguyen TTT,Zhang Y,Shang E,Shu C,Torrini C,Zhao J,Bianchetti E,Mela A,Humala N,Mahajan A,Harmanci AO,Lei Z,Maienschein-Cline M,Quinzii CM,Westhoff MA,Karpel-Massler G,Bruce JN,Canoll P,Siegelin MD

PA5-12217 was used in Immunoelectrophoresis to investigate if glioblastoma (GBM) cultures and patients'' tumors harbor super-enhancers in several genes related to the Warburg effect.

Wed Jul 01 00:00:00 EDT 2020

Loss of macrophage fatty acid oxidation does not potentiate systemic metabolic dysfunction.

American journal of physiology. Endocrinology and metabolism

Gonzalez-Hurtado E,Lee J,Choi J,Selen Alpergin ES,Collins SL,Horton MR,Wolfgang MJ

PA5-12217 was used in Western Blotting to investigate the contribution of macrophage fatty acid oxidation directly to metabolic dysfunction in high-fat diet-induced obesity, by using mice with a myeloid-specific knockout of carnitine palmitoyltransferase II (CPT2 Mϕ-KO).

Mon May 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Carnitine O-palmitoyltransferase 2, mitochondrial; Carnitine palmitoyltransferase II; CPT II; OTTHUMP00000010493; testicular secretory protein Li 13

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Gene Aliases: AI323697; Cpt-2; CPT1; CPT2; CPTASE; CPTII; IIAE4

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UniProt ID: (Human) P23786, (Mouse) P52825

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Entrez Gene ID: (Human) 1376, (Mouse) 12896

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Function(s)

carnitine O-palmitoyltransferase activity transferase activity transferase activity, transferring acyl groups acyltransferase

Process(es)

fatty acid beta-oxidation carnitine shuttle lipid metabolic process fatty acid metabolic process transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CPT2 Polyclonal Antibody

CPT2 Antibody (PA5-12217) in ICC/IF

Product Details

PA5-12217

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoelectrophoresis (IE)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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