Invitrogen

CREB Recombinant Rabbit Monoclonal Antibody (3H8L15)

Advanced Verification

IP-mass spectrometry

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

701120

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunocytochemistry (ICC/IF)

1:500-1:5,000
-

ChIP assay (ChIP)

1 µL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

3H8L15

Immunogen

Recombinant protein corresponding to amino acids 222-341 of human cAMP responsive element binding protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532397

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: active transcription factor CREB; cAMP-response element-binding protein-1; CREB-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein

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Gene Aliases: CREB; CREB-1; CREB1

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UniProt ID: (Human) P16220

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Entrez Gene ID: (Human) 1385

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription cofactor activity protein binding enzyme binding Hsp70 protein binding histone acetyltransferase binding cAMP response element binding identical protein binding arrestin family protein binding
Process(es)
response to hypoxia transcription from RNA polymerase II promoter protein phosphorylation signal transduction transforming growth factor beta receptor signaling pathway axon guidance aging lactation memory circadian rhythm regulation of cell size visual learning response to organic substance negative regulation of transcription by competitive promoter binding response to activity viral process pituitary gland development positive regulation of transforming growth factor beta3 production secretory granule organization response to glucagon chemotaxis to arachidonic acid response to nicotine cellular response to hepatocyte growth factor stimulus cellular response to platelet-derived growth factor stimulus response to antidepressant positive regulation of multicellular organism growth regulation of circadian rhythm positive regulation of apoptotic process positive regulation of fat cell differentiation positive regulation of osteoclast differentiation positive regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone secretion positive regulation of lipid biosynthetic process regulation of fibroblast proliferation protein stabilization regulation of glial cell proliferation lung saccule development Type I pneumocyte differentiation cellular response to zinc ion cellular response to fatty acid positive regulation of long-term synaptic potentiation negative regulation of neuron death response to L-glutamate cellular response to nerve growth factor stimulus cellular response to insulin-like growth factor stimulus
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