Proteintech

CRISP2 Polyclonal Antibody

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Antibody Testing Data (6)

CRISP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

CRISP2 Antibody (19066-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human testis using 19066-1-AP (CRISP2 antibody) at dilution of 1:50 (under 40x lens).

CRISP2 Antibody in Immunoprecipitation (IP)

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Product Details

19066-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CRISP2 Fusion Protein Ag13574 (27-243 aa encoded by BC022011)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TALLTTQLQ VQREIVNKHN ELRKAVSPPA SNMLKMEWSR EVTTNAQRWA NKCTLQHSDP EDRKTSTRCG ENLYMSSDPT SWSSAIQSWY DEILDFVYGV GPKSPNAVVG HYTQLVWYST YQVGCGIAYC PNQDSLKYYY VCQYCPAGNN MNRKNTPYQQ GTPCAGCPDD CDKGLCTNSC QYQDLLSNCD SLKNTAGCEH ELLKEKCKAT CLCENKIY (27-243 aa encoded by BC022011 )

Target Information

The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A male germ-cell-specific ribosome controls male fertility.

Nature

Li H,Huo Y,He X,Yao L,Zhang H,Cui Y,Xiao H,Xie W,Zhang D,Wang Y,Zhang S,Tu H,Cheng Y,Guo Y,Cao X,Zhu Y,Jiang T,Guo X,Qin Y,Sha J

Thu Dec 01 00:00:00 EST 2022

Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa†.

Biology of reproduction

Zhang M,Bromfield EG,Veenendaal T,Klumperman J,Helms JB,Gadella BM

Mon Nov 15 00:00:00 EST 2021

Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa†.

Biology of reproduction

Zhang M,Bromfield EG,Veenendaal T,Klumperman J,Helms JB,Gadella BM

Mon Nov 15 00:00:00 EST 2021

Functional redundancy and compensation: Deletion of multiple murine Crisp genes reveals their essential role for male fertility.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Curci L,Brukman NG,Weigel Muñoz M,Rojo D,Carvajal G,Sulzyk V,Gonzalez SN,Rubinstein M,Da Ros VG,Cuasnicú PS

Tue Dec 01 00:00:00 EST 2020

MicroRNA-27a-mediated repression of cysteine-rich secretory protein 2 translation in asthenoteratozoospermic patients.

Asian journal of andrology

Zhou JH,Zhou QZ,Yang JK,Lyu XM,Bian J,Guo WB,Chen ZJ,Xia M,Xia H,Qi T,Li X,Liu CD

Fri May 04 00:00:00 EDT 2018

PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.

Nature communications

Ding D,Liu J,Dong K,Midic U,Hess RA,Xie H,Demireva EY,Chen C

Tue Oct 10 00:00:00 EDT 2017

PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.

Nature communications

Ding D,Liu J,Dong K,Midic U,Hess RA,Xie H,Demireva EY,Chen C

Tue Oct 10 00:00:00 EDT 2017

Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders.

Molecular human reproduction

Brukman NG,Miyata H,Torres P,Lombardo D,Caramelo JJ,Ikawa M,Da Ros VG,Cuasnicú PS

Fri Apr 01 00:00:00 EDT 2016

Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders.

Molecular human reproduction

Brukman NG,Miyata H,Torres P,Lombardo D,Caramelo JJ,Ikawa M,Da Ros VG,Cuasnicú PS

Fri Apr 01 00:00:00 EDT 2016

The expression of cysteine-rich secretory protein 2 (CRISP2) and its specific regulator miR-27b in the spermatozoa of patients with asthenozoospermia.

Biology of reproduction

Zhou JH,Zhou QZ,Lyu XM,Zhu T,Chen ZJ,Chen MK,Xia H,Wang CY,Qi T,Li X,Liu CD

Thu Jan 01 00:00:00 EST 2015

The expression of cysteine-rich secretory protein 2 (CRISP2) and its specific regulator miR-27b in the spermatozoa of patients with asthenozoospermia.

Biology of reproduction

Zhou JH,Zhou QZ,Lyu XM,Zhu T,Chen ZJ,Chen MK,Xia H,Wang CY,Qi T,Li X,Liu CD

Thu Jan 01 00:00:00 EST 2015

DAZAP1 regulates the splicing of Crem, Crisp2 and Pot1a transcripts.

Nucleic acids research

Chen HY,Yu YH,Yen PH

Fri Nov 01 00:00:00 EDT 2013

DAZAP1 regulates the splicing of Crem, Crisp2 and Pot1a transcripts.

Nucleic acids research

Chen HY,Yu YH,Yen PH

Fri Nov 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Cancer/testis antigen 36; CRISP-2; CT36; Cysteine-rich secretory protein 2; cysteine-rich secretory protein 2-like; glyceraldehyde-3-phosphate dehydrogenase-like 5; MGC111136; OTTHUMP00000016589; testicular tissue protein Li 43; testis specific gene 1; testis specific protein 1; testis specific protein 1 (probe H4-1 p3-1); Testis-specific protein TPX-1

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Gene Aliases: CRISP-2; CRISP2; Crisp2l; CT36; GAPDL5; Tpx-1; TPX1; TSP1

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UniProt ID: (Human) P16562, (Mouse) P16563

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Entrez Gene ID: (Human) 7180, (Mouse) 22024, (Rat) 360445

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Function(s)

defense/immunity protein

Process(es)

single organismal cell-cell adhesion spermatogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CRISP2 Polyclonal Antibody

CRISP2 Antibody (19066-1-AP) in IHC (P)

Product Details

19066-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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