Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
CRP Antibody (500-P242BT-25UG) in ELISA
Sandwich ELISA: To detect Human CRP by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of CRP Polyclonal Antibody, Biotin (Product # 500-P242BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech CRP Polyclonal Antibody (Product # 500-P242-1MG) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human CRP.
Product Details
500-P242BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 23.2 kDa Recombinant Human CRP
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930011
Product Specific Information
AA Sequence of recombinant protein: discontinued.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CRP. Anti-Human CRP-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human CRP by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human CRP (500-P242) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human CRP.
Western Blot: To detect Human CRP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human CRP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P242BT-1MG will be provided as 2 x 500 µg
Target Information
CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein compound consisting of five identical, noncovalently bound, nonglycosylated subunits. Eeach subunit is made up of 206 amino acids, and has a molecular weight of 24 kDa. CRP is produced by the liver, and its plasma levels rise dramatically during inflammatory processes occurring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.), plays an important role in innate immunity, and mediates activities associated with pre-immune nonspecific host resistance. Specifically, there is a 100-1000 fold increase of CRP in response to injury, infection or inflammation. CRP is opsonic, an initiator of the classical complement cascade, and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. CRP is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host, and initiates pathogen elimination by interacting with humoral and cellular effector systems in the blood. Patients with elevated basal levels of CRP have been shown to be an increased risk for hypertension and cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: c reactive; C-reactive protein; CSRP; CYRP; D1S181E; DKFZp686M148; DKFZp686M149; MGC149895; MGC88244; pentraxin 1; RP11-419N10.4
Gene Aliases: CRP; PTX1
UniProt ID: (Human) P02741
Entrez Gene ID: (Human) 1401
acute-phase response
inflammatory response
opsonization
positive regulation of gene expression
negative regulation of macrophage derived foam cell differentiation
negative regulation of lipid storage
positive regulation of superoxide anion generation
negative regulation of vasodilation
defense response to Gram-positive bacterium
regulation of interleukin-8 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support