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CRTH2 Antibody (PA5-88828) in WB
Western blot analysis of CRTH2 in extracts of NIH/3T3 cells. Samples were incubated with CRTH2 Polyclonal antibody (Product # PA5-88828) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88828
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 220-359 of human PTGDR (NP_0009441)
Conjugate
Form
Liquid
Concentration
0.63 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805156
Product Specific Information
Immunogen sequence: GAMRNLYAMH RRLQRHPRSC TRDCAEPRAD GREASPQPLE ELDHLLLLAL MTVLFTMCSL PVIYRAYYGA FKDVKEKNRT SEEAEDLRAL RFLSVISIVD PWIFIIFRSP VFRIFFHKIF IRPLRYRSRC SNSTNMESSL; Positive Samples: NIH/3T3; Cellular Location: Cell membrane, Multi-pass membrane protein
Target Information
CRTH2 (GPR44) is a chemoattractant receptor-homologous molecule expressed on Th2 cells that is an identified receptor for the prostaglandin D2 (PGD2) and prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. CRTH2 is also a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells. CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated: a 3.5 kb transcript expressed primarily in the thalamus, frontal cortex, pons and hippocampus; and a 3.4 kb transcript expressed in fetal liver, leukocytes and thymus. CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions. At least two different isoforms of CRTH2 are known to exist. Single nucleotide polymorphisms in the 3' UTR of the CRTH2 gene have been associated with asthma susceptibility.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD294; chemoattractant receptor homologous molecule expressed on T helper type 2 cells; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G protein-coupled receptor 44; G-protein coupled receptor 44; MGC130436; PGD2 receptor; Prostaglandin D2 receptor 2; prostaglandin D2 receptor CRTH2; putative G-protein coupled receptor 44
Gene Aliases: CD294; CRTH2; DL1R; DP2; GPR44; Grp45; PTGDR2
UniProt ID: (Human) Q9Y5Y4, (Mouse) Q9Z2J6
Entrez Gene ID: (Human) 11251, (Mouse) 14764
chemotaxis
immune response
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
chemical synaptic transmission
calcium-mediated signaling
positive regulation of G-protein coupled receptor protein signaling pathway
negative regulation of male germ cell proliferation
signal transduction
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