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Invitrogen
CSK Recombinant Rabbit Monoclonal Antibody (1H8L6)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
CSK Antibody (703298) in ICC/IF
For immunofluorescence analysis GTL-16 cells were fixed and permeabilized for detection of endogenous CSK using Anti-CSK Recombinant Rabbit Monoclonal Antibody (Product # 703298, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Panel (e-h) shows membra... View More
Product Details
703298
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H8L6
Immunogen
Protein corresponding to human CSK [aa73-aa178]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2762389
Product Specific Information
This antibody is predicted to react with Monkey, Mouse, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
CSK is a tyrosine kinase that has been shown to downregulate the activity of SRC oncoprotein through phosphorylation of its carboxy terminus. Since cell transformation by SRC is caused by various mechanisms that interfere with this phosphorylation, the CSK gene might function as an antioncogene. CSK plays an important role in the regulation of cell growth, differentiation, migration, and immune responses. CSK phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases including LCK, SRC, HCK, FYN, LYN, CSK, and YES1. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft-associated protein PAG (Csk-binding protein).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-Src kinase; MGC117393; Protein-tyrosine kinase CYL; Tyrosine-protein kinase CSK
Gene Aliases: CSK
UniProt ID: (Human) P41240
Entrez Gene ID: (Human) 1445
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
receptor binding
protein binding
ATP binding
protein C-terminus binding
kinase activity
protein phosphatase binding
protein kinase A catalytic subunit binding
identical protein binding
metal ion binding
proline-rich region binding
regulation of cytokine production
adaptive immune response
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
epidermal growth factor receptor signaling pathway
central nervous system development
brain development
negative regulation of cell proliferation
negative regulation of low-density lipoprotein particle clearance
cell migration
cell differentiation
T cell costimulation
negative regulation of interleukin-6 production
negative regulation of kinase activity
adherens junction organization
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
negative regulation of Golgi to plasma membrane protein transport
positive regulation of MAP kinase activity
innate immune response
negative regulation of bone resorption
oligodendrocyte differentiation
negative regulation of phagocytosis
T cell receptor signaling pathway
regulation of Fc receptor mediated stimulatory signaling pathway
negative regulation of ERK1 and ERK2 cascade
cellular response to peptide hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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