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FIGURE: 1 / 1
CST1 Antibody (PA5-121012) in WB
Western blot analysis of CST1 in extracts of various cell lines. Samples were incubated with CST1 Polyclonal antibody (Product # PA5-121012) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-121012
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-141 of human CST1 (NP_001889.2).
Conjugate
Form
Liquid
Concentration
3.75 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914584
Product Specific Information
Positive test controls include: A-431, A-549, BT-474, THP-1. The target is usually found in the following locations: Secreted.
Immunogen sequence: AWSPKEEDRI IPGGIYNADL NDEWVQRALH FAISEYNKAT KDDYYRRPLR VLRARQQTVG GVNYFFDVEV GRTICTKSQP NLDTCAFHEQ PELQKKQLCS FEIYEVPWEN RRSLVKSRCQ ES
Target Information
The cystatin superfamily is a well-established family of cysteine protease inhibitors. Cystatins A and B (type 1) are mainly intracellular; cystatins C, D, E/M, F, G, S, SN and SA cystatins are extracellular (type 2); and the kininogens are type 3 cystatins which are intravascular proteins. All true cystatins inhibit cysteine peptidases of the papain family, such as cathepsins, and some also inhibit legumain family enzymes. Cystatin SA, cystatin S and cystatin SN are found primarily in saliva. Cystatin S and SN can also be expressed in tears, urine and seminal fluid. Cystatin C is a related protein which is expressed in brain, thymus, ovary, epididymis and vas deferens. Cystatin D protects against proteinases in the oral cavity, while Cystatin E/M and F moderate the inhibition of cathepsin proteins. The fetuins, part of the cystatin superfamily, are secretable proteins that influence osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors and the response to systemic inflammation. High molecular weight kininogen (Kininogen HC) and low molecular weight kininogen (Kininogen LC) have varied roles, though they both inhibit the thrombin- and plasmin-induced aggregation of thrombocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cystain-SA-I; cystatin 1; cystatin SA-I; Cystatin-1; Cystatin-SN; cysteine proteinase inhibitor, type 2 family; Salivary cystatin-SA-1
Gene Aliases: CST1
UniProt ID: (Human) P01037
Entrez Gene ID: (Human) 1469
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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