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CX3CR1 (extracellular) Antibody (ACR-058-F-50UL) in Flow
Cell surface detection of CX3CR1 by direct flow cytometry in live intact mouse J774 macrophage cells: - (black line) cells. (red) Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F). (green) Cells + Anti-CX3CR1 (extracellular)-FITC Antibody (#ACR-058-F), (2.5 µg).
Product Details
ACR-058-F-50UL
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)ELDLENFEYDDSAE, corresponding to amino acid residues 7- 20 of mouse CX3CR1, Extracellular, N-terminus
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 15 µL or 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration an d adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-X3-C CKR-1; chemokine (C-X3-C) receptor 1; Cx3c; CX3C chemokine receptor 1; Fractalkine receptor; Scyd1
Gene Aliases: Cx3cr1; Rbs11
UniProt ID: (Rat) P35411, (Mouse) Q9Z0D9
Entrez Gene ID: (Rat) 171056, (Mouse) 13051
microglial cell activation
positive regulation of neuroblast proliferation
microglial cell activation involved in immune response
negative regulation of chronic inflammatory response to non-antigenic stimulus
cell adhesion
G-protein coupled receptor signaling pathway
memory
negative regulation of angiogenesis
cytokine-mediated signaling pathway
cerebral cortex cell migration
negative regulation of cell migration
positive regulation of angiogenesis
macrophage chemotaxis
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to transforming growth factor beta stimulus
negative regulation of long-term synaptic potentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
chemotaxis
signal transduction
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