Invitrogen

CXCL1 Polyclonal Antibody, PeproTech®

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CXCL1 Antibody (500-P92-1MG) in ELISA

Sandwich ELISA: To detect Human GRO-alpha/MGSA (CXCL1) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CXCL1 Polyclonal Antibody (Product # 500-P92-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CXCL1 Polyclonal Antibody, Biotin (Product # 500-P92BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-alpha/MGSA (CXCL1).

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Product Details

500-P92-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 2 publications 2 publications

In vitro Assay (IV)

View 1 publication 1 publication

Immunostaining (IS)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human GRO-alpha/MGSA (CXCL1)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930858

Product Specific Information

AA Sequence of recombinant protein: ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-alpha/MGSA (CXCL1). Anti-Human GRO-alpha/MGSA (CXCL1)-specific antibody was purified by affinity chromatography employing an immobilized Human GRO-alpha/MGSA (CXCL1) matrix.

Sandwich ELISA: To detect Human GRO-alpha/MGSA (CXCL1) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GRO-alpha/MGSA (CXCL1) (500-P92Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-alpha/MGSA (CXCL1).

Western Blot: To detect Human GRO-alpha/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human GRO-alpha/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Global Phosphorylation Landscape of SARS-CoV-2 Infection.

Cell

Bouhaddou M,Memon D,Meyer B,White KM,Rezelj VV,Correa Marrero M,Polacco BJ,Melnyk JE,Ulferts S,Kaake RM,Batra J,Richards AL,Stevenson E,Gordon DE,Rojc A,Obernier K,Fabius JM,Soucheray M,Miorin L,Moreno E,Koh C,Tran QD,Hardy A,Robinot R,Vallet T,Nilsson-Payant BE,Hernandez-Armenta C,Dunham A,Weigang S,Knerr J,Modak M,Quintero D,Zhou Y,Dugourd A,Valdeolivas A,Patil T,Li Q,Hüttenhain R,Cakir M,Muralidharan M,Kim M,Jang G,Tutuncuoglu B,Hiatt J,Guo JZ,Xu J,Bouhaddou S,Mathy CJP,Gaulton A,Manners EJ,Félix E,Shi Y,Goff M,Lim JK,McBride T,O'Neal MC,Cai Y,Chang JCJ,Broadhurst DJ,Klippsten S,De Wit E,Leach AR,Kortemme T,Shoichet B,Ott M,Saez-Rodriguez J,tenOever BR,Mullins RD,Fischer ER,Kochs G,Grosse R,García-Sastre A,Vignuzzi M,Johnson JR,Shokat KM,Swaney DL,Beltrao P,Krogan NJ

500-P92 was used in Enzyme-linked immunosorbent assay to present a quantitative mass spectrometry-based phosphoproteomics survey of SARS-CoV-2 infection in Vero E6 cells, revealing dramatic rewiring of phosphorylation on host and viral proteins.

Thu Aug 06 00:00:00 EDT 2020

Statins affect the presentation of endothelial chemokines by targeting to multivesicular bodies.

PloS one

Hol J,Otterdal K,Breland UM,Stang E,Pedersen TM,Hagelsteen K,Ranheim T,Kasprzycka M,Halvorsen B,Haraldsen G,Aukrust P

Thu Mar 21 00:00:00 EDT 2013

Glyoxalase-I is a novel prognosis factor associated with gastric cancer progression.

PloS one

Cheng WL,Tsai MM,Tsai CY,Huang YH,Chen CY,Chi HC,Tseng YH,Chao IW,Lin WC,Wu SM,Liang Y,Liao CJ,Lin YH,Chung IH,Chen WJ,Lin PY,Wang CS,Lin KH

Mon Jul 30 00:00:00 EDT 2012

A revised model for the secretion of tPA and cytokines from cultured endothelial cells.

Blood

Knipe L,Meli A,Hewlett L,Bierings R,Dempster J,Skehel P,Hannah MJ,Carter T

Thu Sep 23 00:00:00 EDT 2010

Endothelium-derived but not platelet-derived protein disulfide isomerase is required for thrombus formation in vivo.

Blood

Jasuja R,Furie B,Furie BC

Thu Nov 25 00:00:00 EST 2010

Characterization of a novel chemokine-containing storage granule in endothelial cells: evidence for preferential exocytosis mediated by protein kinase A and diacylglycerol.

Journal of immunology (Baltimore, Md. : 1950)

Øynebråten I,Barois N,Hagelsteen K,Johansen FE,Bakke O,Haraldsen G

Sat Oct 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CXC; CXC motif chemokine; CXCL; CXCL-1; fibroblast secretory protein; GRO a; GRO α; GRO-alpha(1-73); GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3

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Gene Aliases: CXCL1; FSP; GRO; GRO1; GROA; MGSA; MGSA-a; NAP-3; SCYB1

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UniProt ID: (Human) P09341

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Entrez Gene ID: (Human) 2919

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Function(s)

receptor binding chemokine activity enzyme activator activity growth factor activity CXCR chemokine receptor binding chemokine

Process(es)

chemotaxis inflammatory response immune response signal transduction G-protein coupled receptor signaling pathway nervous system development cell proliferation negative regulation of cell proliferation actin cytoskeleton organization response to lipopolysaccharide intracellular signal transduction positive regulation of catalytic activity cell chemotaxis chemokine-mediated signaling pathway positive regulation of neutrophil chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CXCL1 Polyclonal Antibody, PeproTech®

CXCL1 Antibody (500-P92-1MG) in ELISA

Product Details

500-P92-1MG

Western Blot (WB)

ELISA (ELISA)

In vitro Assay (IV)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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