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CXCL1 (GRO alpha) Antibody (PA5-79102) in WB
Western blot analysis of CXCL1 (GRO alpha) in Lane 1: recombinant rat CXCL1 protein using 1 ng per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with CXCL1 (GRO alpha) polyclonal antibody (Product # PA5-79102) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP seconda... View More
Product Details
PA5-79102
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat GRO alpha recombinant protein (Position: A25-K96).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2746218
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; gro; GRO a; GRO α; GRO1 oncogene; GROalpha; Growth-regulated alpha protein; GROα; KC/GR)-alpha; KC/GRO-alpha; NAP3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51
Gene Aliases: CINC-1; Cinc1; Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1
UniProt ID: (Rat) P14095, (Mouse) P12850
Entrez Gene ID: (Rat) 81503, (Mouse) 14825
response to amphetamine
response to molecule of bacterial origin
acute inflammatory response
positive regulation of leukocyte chemotaxis
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
response to heat
response to gamma radiation
positive regulation of sodium ion transport
neutrophil chemotaxis
response to estradiol
response to lipopolysaccharide
positive regulation of superoxide anion generation
regulation of cell proliferation
positive regulation of potassium ion transport
response to glucocorticoid
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of neutrophil mediated killing of fungus
positive regulation of hematopoietic stem cell proliferation
inflammatory response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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