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CXCL10 Antibody (500-P129BT-25UG) in ELISA
Sandwich ELISA: To detect Murine IP-10 (CXCL10) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of CXCL10 Polyclonal Antibody, Biotin (Product # 500-P129BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech CXCL10 Polyclonal Antibody (Product # 500-P129-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IP-10 (CXCL10).
Product Details
500-P129BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IP-10 (CXCL10)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929376
Product Specific Information
AA Sequence of recombinant protein: IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IP-10 (CXCL10). Anti-Murine IP-10 (CXCL10)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine IP-10 (CXCL10) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IP-10 (CXCL10) (500-P129) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IP-10 (CXCL10).
Western Blot: To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P129BT-1MG will be provided as 2 x 500 µg
Target Information
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 10 kDa interferon gamma-induced protein; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 10; C7; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; Gamma-IP10; H-IP-10; interferon activated gene 10; Interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; M-IP-10; small inducible cytokine B subfamily (Cys-X-Cys), member 10; Small-inducible cytokine B10
Gene Aliases: C7; CRG-2; Crg2; Cxcl10; gIP-10; Ifi10; INP10; IP-10; IP10; mob-1; Scyb10
UniProt ID: (Mouse) P17515
Entrez Gene ID: (Mouse) 15945
positive regulation of leukocyte chemotaxis
chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of cell proliferation
T cell chemotaxis
regulation of T cell chemotaxis
negative regulation of angiogenesis
positive regulation of cell migration
positive regulation of cAMP metabolic process
response to lipopolysaccharide
endothelial cell activation
regulation of cell proliferation
positive regulation of cAMP-mediated signaling
negative regulation of myoblast differentiation
positive regulation of release of sequestered calcium ion into cytosol
defense response to virus
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
positive regulation of monocyte chemotaxis
regulation of endothelial tube morphogenesis
negative regulation of myoblast fusion
positive regulation of T cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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