Invitrogen

CXCL12 Monoclonal Antibody (79018)

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (5)

FIGURE: 1 / 5

CXCL12 Antibody (MA5-23759) in WB

Nature communications 2022 - Expansion and accumulations of MDSCs in Brca1 MT in vivo and in vitro. a - c Migration assay of MDSCs from spleens of WT ( a ) and Brca1-MT ( b ) mice in conditional mediums (CM), including CMs from B477 cells (Ctr-B477), OE- S100a9 in B477 cells (OE-S 100a9 -B477), G600 cells (Ctr-G600), and G600 cells expressing S100a9 (sg- S100a9 -G600). Quantification c in ( a and b , Scale bar: 0.2 mm, n = 16-19 pictur... View More

CXCL12 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

MA5-23759

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

8-25 µg/mL

Immunocytochemistry (ICC/IF)

8-15 µg/mL

Flow Cytometry (Flow)

0.25 µg per million cells

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

79018

Immunogen

E. coli-derived recombinant human CXCL12/SDF-1 alpha Lys22-Lys89

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2608711

Product Specific Information

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Single-Cell RNA-Sequencing Reveals Lineage-Specific Regulatory Changes of Fibroblasts and Vascular Endothelial Cells in Keloids.

The Journal of investigative dermatology

Liu X,Chen W,Zeng Q,Ma B,Li Z,Meng T,Chen J,Yu N,Zhou Z,Long X

MA5-23759 was used in Immunohistochemistry-immunofluorescence to highlight the roles of transforming growth factor beta and Eph-ephrin signaling pathways in both the aberrant fibrogenesis and angiogenesis of keloids.

Sat Jan 01 00:00:00 EST 2022

CXCL12/CXCR4 axis supports mitochondrial trafficking in tumor myeloma microenvironment.

Oncogenesis

Giallongo C,Dulcamare I,Tibullo D,Del Fabro V,Vicario N,Parrinello N,Romano A,Scandura G,Lazzarino G,Conticello C,Li Volti G,Amorini AM,Musumeci G,Di Rosa M,Polito F,Oteri R,Aguennouz M,Parenti R,Di Raimondo F,Palumbo GA

MA5-23759 was used in Western Blot to demonstrate that myeloma mesenchymal stromal cells (MSCs) have decreased reliance on mitochondrial metabolism as compared to healthy MSCs and increased tendency to deliver mitochondria to multiple myeloma cells.

Fri Jan 21 00:00:00 EST 2022

CXCL12/CXCR4 axis supports mitochondrial trafficking in tumor myeloma microenvironment.

Oncogenesis

Fri Jan 21 00:00:00 EST 2022

Elevated CXCL12 in the plasma membrane of locally advanced rectal cancer after neoadjuvant chemoradiotherapy: a potential prognostic marker.

Journal of Cancer

Kim S,Yeo MK,Kim JS,Kim JY,Kim KH

MA5-23759 was used in Immunohistochemistry to suggest that CXCL12 expression in the plasma membrane of radioresistant LARC cells may be a predictive factor of recurrence and a viable therapeutic strategy to control radioresistant LARC recurrence.

Tue Jan 04 00:00:00 EST 2022

Elevated CXCL12 in the plasma membrane of locally advanced rectal cancer after neoadjuvant chemoradiotherapy: a potential prognostic marker.

Journal of Cancer

Kim S,Yeo MK,Kim JS,Kim JY,Kim KH

Tue Jan 04 00:00:00 EST 2022

S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy.

Nature communications

Li J,Shu X,Xu J,Su SM,Chan UI,Mo L,Liu J,Zhang X,Adhav R,Chen Q,Wang Y,An T,Zhang X,Lyu X,Li X,Lei JH,Miao K,Sun H,Xing F,Zhang A,Deng C,Xu X

MA5-23759 was used in Western Blot to provide a selective strategy for effective immunotherapy in patients with elevated S100A9 and/or CXCL12 protein levels.

Fri Mar 18 00:00:00 EDT 2022

S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy.

Nature communications

Li J,Shu X,Xu J,Su SM,Chan UI,Mo L,Liu J,Zhang X,Adhav R,Chen Q,Wang Y,An T,Zhang X,Lyu X,Li X,Lei JH,Miao K,Sun H,Xing F,Zhang A,Deng C,Xu X

MA5-23759 was used in Western Blot to provide a selective strategy for effective immunotherapy in patients with elevated S100A9 and/or CXCL12 protein levels.

Fri Mar 18 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; AI174028; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; CXC; CXC motif chemokine; CXCL; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; Pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; SDF-1; SDF-1a; SDF-1b; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; Thymic lymphoma cell-stimulating factor; TLSF; TLSF-a; TLSF-b; Tlsfa; Tlsfb; TPAR1

View more View less

Gene Aliases: CXCL12; IRH; PBSF; SCYB12; SDF1; SDF1A; SDF1B; TLSF; TPAR1

View more View less

UniProt ID: (Human) Q5IT36, (Mouse) P40224

View more View less

Entrez Gene ID: (Human) 6387, (Mouse) 20315

View more View less

Function(s)

receptor binding chemokine activity growth factor activity chemoattractant activity chemokine receptor binding CXCR chemokine receptor binding cytokine activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CXCL12 Monoclonal Antibody (79018)

CXCL12 Antibody (MA5-23759) in WB

Product Details

MA5-23759

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!