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CXCL12 alpha (SDF-1 alpha) Antibody (500-P164GBT-25UG) in ELISA
Sandwich ELISA: To detect Murine SDF-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin (Product # 500-P164GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody (Product # 500-P164G-1MG) as a capture antibody, allows the d... View More
Product Details
500-P164GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 7.9 kDa Recombinant Murine SDF-1alpha (CXCL12)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929585
Product Specific Information
AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK.
Preparation: Produced from sera of goats immunized with highly pure recombinant Murine SDF-1alpha (CXCL12). Anti-Murine SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine SDF-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine SDF-1alpha (500-P164G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine SDF-1alpha.
Western Blot: To detect Murine SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P164GBT-1MG will be provided as 2 x 500 µg
Target Information
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; H-SDF-1-alpha; PBSF; Pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; SDF 1 a; SDF 1 alpha; SDF 1α; SDF-1; SDF1 alpha; SDF1α; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; Thymic lymphoma cell-stimulating factor; TLSF; TPAR1
Gene Aliases: Cxcl12; Pbsf; Scyb12; Sdf1; Tlsf; Tpar1
UniProt ID: (Mouse) P40224
Entrez Gene ID: (Mouse) 20315
patterning of blood vessels
ameboidal-type cell migration
neuron migration
positive regulation of endothelial cell proliferation
chemotaxis
immune response
germ cell development
brain development
motor neuron axon guidance
positive regulation of cell proliferation
adult locomotory behavior
germ cell migration
telencephalon cell migration
regulation of cell migration
positive regulation of cell migration
organ regeneration
positive regulation of dopamine secretion
T cell proliferation
positive regulation of neuron differentiation
positive regulation of cell adhesion
positive regulation of axon extension involved in axon guidance
induction of positive chemotaxis
regulation of calcium ion transport
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of monocyte chemotaxis
positive regulation of myoblast fusion
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of leukocyte tethering or rolling
negative regulation of leukocyte apoptotic process
positive regulation of T cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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