Invitrogen

CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech®

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Antibody Testing Data (3)

CXCL12 alpha (SDF-1 alpha) Antibody in ELISA (ELISA)

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CXCL12 alpha (SDF-1 alpha) Antibody (500-P87A-1MG) in ELISA

Sandwich ELISA: To detect Human SDF-1alpha (CXCL12) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody (Product # 500-P87A-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin (Product # 500-P87ABT-1MG) as a detection antibody, all... View More

CXCL12 alpha (SDF-1 alpha) Antibody in Western Blot (WB)

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Product Details

500-P87A-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 3 publications 3 publications

Neutralization (Neu)

View 2 publications 2 publications

In vitro Assay (IV)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Immunostaining (IS)

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human SDF-1alpha (CXCL12)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930822

Product Specific Information

AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human SDF-1alpha (CXCL12). Anti-Human SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography employing an immobilized Human SDF-1alpha (CXCL12) matrix.

Sandwich ELISA: To detect Human SDF-1alpha (CXCL12) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human SDF-1alpha (CXCL12) (500-P87ABt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human SDF-1alpha (CXCL12).

Western Blot: To detect Human SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human SDF-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Surface functionalization of electrospun scaffolds using recombinant human decorin attracts circulating endothelial progenitor cells.

Scientific reports

Hinderer S,Sudrow K,Schneider M,Holeiter M,Layland SL,Seifert M,Schenke-Layland K

Mon Jan 08 00:00:00 EST 2018

Surface functionalization of electrospun scaffolds using recombinant human decorin attracts circulating endothelial progenitor cells.

Scientific reports

Hinderer S,Sudrow K,Schneider M,Holeiter M,Layland SL,Seifert M,Schenke-Layland K

Mon Jan 08 00:00:00 EST 2018

Chemokine axes in breast cancer: factors of the tumor microenvironment reshape the CCR7-driven metastatic spread of luminal-A breast tumors.

Journal of leukocyte biology

Weitzenfeld P,Kossover O,Körner C,Meshel T,Wiemann S,Seliktar D,Legler DF,Ben-Baruch A

500-P87A was used in Enzyme-linked immunosorbent assay to study the importance of chemokine axes in regulating the metastatic spread of breast cancer.

Wed Jun 01 00:00:00 EDT 2016

CXCL12 mediates trophic interactions between endothelial and tumor cells in glioblastoma.

PloS one

Rao S,Sengupta R,Choe EJ,Woerner BM,Jackson E,Sun T,Leonard J,Piwnica-Worms D,Rubin JB

Mon Aug 20 00:00:00 EDT 2012

The chemokine CXCL12 regulates monocyte-macrophage differentiation and RUNX3 expression.

Blood

Sánchez-Martín L,Estecha A,Samaniego R,Sánchez-Ramón S,Vega MÁ,Sánchez-Mateos P

Thu Jan 06 00:00:00 EST 2011

CXCR7 influences leukocyte entry into the CNS parenchyma by controlling abluminal CXCL12 abundance during autoimmunity.

The Journal of experimental medicine

Cruz-Orengo L,Holman DW,Dorsey D,Zhou L,Zhang P,Wright M,McCandless EE,Patel JR,Luker GD,Littman DR,Russell JH,Klein RS

Mon Feb 14 00:00:00 EST 2011

Intravenous administration of mesenchymal stem cells exerts therapeutic effects on parkinsonian model of rats: focusing on neuroprotective effects of stromal cell-derived factor-1alpha.

BMC neuroscience

Wang F,Yasuhara T,Shingo T,Kameda M,Tajiri N,Yuan WJ,Kondo A,Kadota T,Baba T,Tayra JT,Kikuchi Y,Miyoshi Y,Date I

Mon Apr 26 00:00:00 EDT 2010

IL-1R signaling within the central nervous system regulates CXCL12 expression at the blood-brain barrier and disease severity during experimental autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

McCandless EE,Budde M,Lees JR,Dorsey D,Lyng E,Klein RS

Wed Jul 01 00:00:00 EDT 2009

Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling.

Proceedings of the National Academy of Sciences of the United States of America

Hay DC,Fletcher J,Payne C,Terrace JD,Gallagher RC,Snoeys J,Black JR,Wojtacha D,Samuel K,Hannoun Z,Pryde A,Filippi C,Currie IS,Forbes SJ,Ross JA,Newsome PN,Iredale JP

Tue Aug 26 00:00:00 EDT 2008

Blocking CXCR4-mediated cyclic AMP suppression inhibits brain tumor growth in vivo.

Cancer research

Yang L,Jackson E,Woerner BM,Perry A,Piwnica-Worms D,Rubin JB

Mon Jan 15 00:00:00 EST 2007

Blocking CXCR4-mediated cyclic AMP suppression inhibits brain tumor growth in vivo.

Cancer research

Yang L,Jackson E,Woerner BM,Perry A,Piwnica-Worms D,Rubin JB

Mon Jan 15 00:00:00 EST 2007

Shedding of syndecan-1 by stromal fibroblasts stimulates human breast cancer cell proliferation via FGF2 activation.

The Journal of biological chemistry

Su G,Blaine SA,Qiao D,Friedl A

Fri May 18 00:00:00 EDT 2007

CXCL12 limits inflammation by localizing mononuclear infiltrates to the perivascular space during experimental autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

McCandless EE,Wang Q,Woerner BM,Harper JM,Klein RS

Fri Dec 01 00:00:00 EST 2006

Identification of carboxypeptidase N as an enzyme responsible for C-terminal cleavage of stromal cell-derived factor-1alpha in the circulation.

Blood

Davis DA,Singer KE,De La Luz Sierra M,Narazaki M,Yang F,Fales HM,Yarchoan R,Tosato G

Wed Jun 15 00:00:00 EDT 2005

Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.

Blood

Piovan E,Tosello V,Indraccolo S,Cabrelle A,Baesso I,Trentin L,Zamarchi R,Tamamura H,Fujii N,Semenzato G,Chieco-Bianchi L,Amadori A

Tue Feb 01 00:00:00 EST 2005

Differential processing of stromal-derived factor-1alpha and stromal-derived factor-1beta explains functional diversity.

Blood

De La Luz Sierra M,Yang F,Narazaki M,Salvucci O,Davis D,Yarchoan R,Zhang HH,Fales H,Tosato G

Thu Apr 01 00:00:00 EST 2004

Selective expression of stromal-derived factor-1 in the capillary vascular endothelium plays a role in Kaposi sarcoma pathogenesis.

Blood

Yao L,Salvucci O,Cardones AR,Hwang ST,Aoki Y,De La Luz Sierra M,Sajewicz A,Pittaluga S,Yarchoan R,Tosato G

Mon Dec 01 00:00:00 EST 2003

Regulation of endothelial cell branching morphogenesis by endogenous chemokine stromal-derived factor-1.

Blood

Salvucci O,Yao L,Villalba S,Sajewicz A,Pittaluga S,Tosato G

Mon Apr 15 00:00:00 EDT 2002

Regional expression of CXCL12/CXCR4 in liver and hepatocellular carcinoma and cell-cycle variation during in vitro differentiation.

Japanese journal of cancer research : Gann

Shibuta K,Mori M,Shimoda K,Inoue H,Mitra P,Barnard GF

Mon Jul 01 00:00:00 EDT 2002

Chemoattractants induce a rapid and transient upregulation of monocyte alpha4 integrin affinity for vascular cell adhesion molecule 1 which mediates arrest: an early step in the process of emigration.

The Journal of experimental medicine

Chan JR,Hyduk SJ,Cybulsky MI

Mon May 21 00:00:00 EDT 2001

Role of tyrosine phosphorylation in ligand-independent sequestration of CXCR4 in human primary monocytes-macrophages.

The Journal of biological chemistry

Wang J,Guan E,Roderiquez G,Calvert V,Alvarez R,Norcross MA

Fri Dec 28 00:00:00 EST 2001

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; CXC; CXC motif chemokine; CXCL; H-SDF-1-alpha; Intercrine reduced in hepatomas; IRH; PBSF; Pre-B cell growth-stimulating factor; SDF 1 a; SDF 1 alpha; SDF 1α; SDF-1; SDF1 alpha; SDF1α; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha

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Gene Aliases: CXCL12; IRH; PBSF; SCYB12; SDF1; SDF1A; SDF1B; TLSF; TPAR1

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UniProt ID: (Human) P48061

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Entrez Gene ID: (Human) 6387

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Function(s)

receptor binding chemokine activity growth factor activity chemoattractant activity chemokine receptor binding CXCR chemokine receptor binding

Process(es)

response to hypoxia neuron migration positive regulation of endothelial cell proliferation cellular calcium ion homeostasis chemotaxis immune response cell adhesion signal transduction G-protein coupled receptor signaling pathway axon guidance blood circulation regulation of actin polymerization or depolymerization adult locomotory behavior response to radiation response to heat response to mechanical stimulus response to virus telencephalon cell migration organ regeneration positive regulation of dopamine secretion response to peptide hormone positive regulation of cell adhesion positive regulation of axon extension involved in axon guidance positive chemotaxis induction of positive chemotaxis cell chemotaxis chemokine-mediated signaling pathway positive regulation of monocyte chemotaxis positive regulation of calcium ion import negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of leukocyte tethering or rolling negative regulation of leukocyte apoptotic process positive regulation of T cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech®

CXCL12 alpha (SDF-1 alpha) Antibody (500-P87A-1MG) in ELISA

Product Details

500-P87A-1MG

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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