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CXCL14 Antibody (500-P237BT-25UG) in WB
Western Blot: To detect hBRAK by Western Blot analysis CXCL14 Polyclonal Antibody, Biotin (Product # 500-P237BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBRAK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P237BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human BRAK (CXCL14)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929976
Product Specific Information
AA Sequence of recombinant protein: SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BRAK (CXCL14). Anti-Human BRAK (CXCL14)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBRAK by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BRAK (500-P237) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBRAK.
Western Blot: To detect hBRAK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBRAK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P237BT-1MG will be provided as 2 x 500 µg
Target Information
This gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bolekine; breast and kidney; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 14; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXC; CXC chemokine in breast and kidney; CXC motif chemokine; CXCL; MGC10687; MIP-2 gamma; MIP-2G; small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK); Small-inducible cytokine B14; tumor-suppressing chemokine
Gene Aliases: BMAC; BRAK; CXCL14; KEC; KS1; MIP-2g; MIP2G; NJAC; PSEC0212; SCYB14; UNQ240/PRO273
UniProt ID: (Human) O95715
Entrez Gene ID: (Human) 9547
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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