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CXCL2 Antibody (500-P130BT-25UG) in WB
Western Blot: To detect mMIP-2 by Western Blot analysis CXCL2 Polyclonal Antibody, Biotin (Product # 500-P130BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMIP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P130BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine MIP-2 (CXCL2)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929385
Product Specific Information
AA Sequence of recombinant protein: AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-2 (CXCL2). Anti-Murine MIP-2 (CXCL2)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine MIP-2 (CXCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MIP-2 (CXCL2) (500-P130) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-2 (CXCL2).
Western Blot: To detect mMIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMIP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P130BT-1MG will be provided as 2 x 500 µg
Target Information
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 2; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; GRO b; gro beta; GRO β; GRObeta; GROβ; Macrophage inflammatory protein 2; MIP 2 a; MIP 2 alpha; MIP 2 α; MIP2; MIP2 a; MIP2 α; small inducible cytokine subfamily, member 2
Gene Aliases: CINC-2a; Cxcl2; Gro2; GROb; Mgsa-b; MIP-2; MIP-2a; Mip2; Scyb; Scyb2
UniProt ID: (Mouse) P10889
Entrez Gene ID: (Mouse) 20310
response to molecule of bacterial origin
positive regulation of leukocyte chemotaxis
chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
neutrophil chemotaxis
leukocyte chemotaxis
response to lipopolysaccharide
regulation of cell proliferation
cell chemotaxis
chemokine-mediated signaling pathway
neuron death
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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