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CXCL3 Antibody (500-P105BT-25UG) in ELISA
Sandwich ELISA: To detect Human GRO-gamma by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of CXCL3 Polyclonal Antibody, Biotin (Product # 500-P105BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech CXCL3 Polyclonal Antibody (Product # 500-P105-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-gamma.
Product Details
500-P105BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human GRO-gamma (CXCL3)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929214
Product Specific Information
AA Sequence of recombinant protein: ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-gamma (CXCL3). Anti-Human GRO-gamma (CXCL3)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human GRO-gamma by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GRO-gamma (500-P105) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-gamma.
Western Blot: To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P105BT-1MG will be provided as 2 x 500 µg
Target Information
CXCL3 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 3; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 3; CXC; CXC motif chemokine; CXCL; gro gamma; GRO-gamma; GRO-gamma(1-73); GRO3 oncogene; Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2-beta
Gene Aliases: CINC-2b; CXCL3; GRO3; GROG; MIP-2b; MIP2B; SCYB3
UniProt ID: (Human) P19876
Entrez Gene ID: (Human) 2921
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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