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CXCL3 Antibody (PA5-47704) in Neu
Neutralization of CXCL3 in BaF3 mouse pro‚B cell line transfected with human CXCR2. Samples were incubated in CXCL3 polyclonal antibody (Product # PA5-47704). Recombinant Rat CXCL3/CINC‚2α chemoattracts the BaF3 mouse pro‚B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Rat CXCL3/CINC‚2α (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat... View More
Product Details
PA5-47704
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant rat CXCL3/CINC-2 alpha/beta Arg33-Ser100
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608020
Product Specific Information
In direct ELISAs, greater than 50% cross-reactivity with recombinant rat (rr) CINC-1, rrCINC-2 beta, and rrCINC-3 is observed, less than 25% cross-reactivity with recombinant mouse (rm) KC, rmMIP-2, recombinant human (rh) GRO alpha, rhGRO beta, and rhGRO gamma is observed, and less than 10% cross-reactivity with rhIL-8 is observed.
Reconstitute at 0.1 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
CXCL3 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 3; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; cytokine-induced neutrophil chemoattractant 2; cytokine-induced neutrophil chemoattractant-2; gro gamma; macrophage inflammatory protein 2-alpha/beta; MIP2-alpha/beta
Gene Aliases: Cinc-2; Cinc2; Gm1960
Entrez Gene ID: (Rat) 171551
positive regulation of leukocyte chemotaxis
chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
hemopoiesis
neutrophil chemotaxis
response to lipopolysaccharide
regulation of cell proliferation
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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