Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
CXCL5 Antibody (500-P91BT-25UG) in ELISA
Sandwich ELISA: To detect hENA-78 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of CXCL5 Polyclonal Antibody, Biotin (Product # 500-P91BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech CXCL5 Polyclonal Antibody (Product # 500-P91-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hENA-78.
Product Details
500-P91BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human ENA-78 (CXCL5) (5-78 a.a.)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930855
Product Specific Information
AA Sequence of recombinant protein: AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human ENA-78 (CXCL5). Anti-Human ENA-78 (CXCL5)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hENA-78 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human ENA-78 (500-P91) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hENA-78.
Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P91BT-1MG will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 5; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 5; CXC; CXC motif chemokine; CXCL; ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); Small-inducible cytokine B5
Gene Aliases: CXCL5; ENA-78; ENA78; SCYB5
UniProt ID: (Human) P42830
Entrez Gene ID: (Human) 6374
neutrophil mediated immunity
positive regulation of leukocyte chemotaxis
chemotaxis
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
positive regulation of cell proliferation
response to lipopolysaccharide
cell chemotaxis
chemokine-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support