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CXCL6 Antibody (500-P120-1MG) in ELISA
Sandwich ELISA: To detect Human GCP-2 (CXCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CXCL6 Polyclonal Antibody (Product # 500-P120-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CXCL6 Polyclonal Antibody, Biotin (Product # 500-P120BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GCP-2 (CXCL6).
Product Details
500-P120-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human GCP-2 (CXCL6)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929316
Product Specific Information
AA Sequence of recombinant protein: VLTELRCTCL RVTLRVNPKT IGKLQVFPAG PQCSKVEVVA SLKNGKQVCL DPEAPFLKKV IQKILDSGNK KN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GCP-2 (CXCL6). Anti-Human GCP-2 (CXCL6) specific antibody was purified by affinity chromatography employing an immobilized Human GCP-2 matrix.
Sandwich ELISA: To detect Human GCP-2 (CXCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GCP-2 (CXCL6)(500-P120Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GCP-2 (CXCL6).
Western Blot: To detect Human GCP-2 (CXCL6) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GCP-2 (CXCL6) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 6; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3; CKA3; CXC; CXC motif chemokine; CXCL; GCP-2; Granulocyte chemotactic protein 2; small inducible cytokine subfamily B (Cys-X-Cys), member 6 (granulocyte chemotactic protein 2); Small inducible cytokine subfamily B (Cys-X-Cys), member b; Small-inducible cytokine B6
Gene Aliases: CKA-3; CXCL6; GCP-2; GCP2; SCYB6
UniProt ID: (Human) P80162
Entrez Gene ID: (Human) 6372
neutrophil mediated immunity
positive regulation of leukocyte chemotaxis
chemotaxis
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
response to lipopolysaccharide
regulation of cell proliferation
defense response to bacterium
cell chemotaxis
chemokine-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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