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CXCL6 Antibody (500-P146-1MG) in WB
Western Blot: To detect Murine LIX (CXCL6) by Western Blot analysis CXCL6 Polyclonal Antibody (Product # 500-P146-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine LIX (CXCL6) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P146-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse,
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine LIX (CXCL6) (92 a.a.)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929478
Product Specific Information
AA Sequence of recombinant protein: APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Mutine LIX (CXCL6). Anti-Murine LIX (CXCL6)-specific antibody was purified by affinity chromatography employing an immobilized Murine LIX (CXCL6) matrix.
Sandwich ELISA: To detect Murine LIX (CXCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine LIX (CXCL6) (500-P146Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine LIX (CXCL6).
Western Blot: To detect Murine LIX (CXCL6) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine LIX (CXCL6) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CKA3; CXC; CXC motif chemokine; CXCL
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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