Proteintech

CXCL8/IL-8 Polyclonal Antibody

CXCL8/IL-8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CXCL8/IL-8 Antibody (17038-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human tonsilitis tissue slide using 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CXCL8/IL-8 Antibody in Western Blot (WB)

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Product Details

17038-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CXCL8,IL8 Fusion Protein Ag10552

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

300 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mesenchymal stem cells derived from adipose accelerate the progression of colon cancer by inducing a MT-CAFs phenotype via TRPC3/NF-KB axis.

Stem cell research & therapy

Xue C,Gao Y,Li X,Zhang M,Yang Y,Han Q,Sun Z,Bai C,Zhao RC

Sat Jul 23 00:00:00 EDT 2022

IL-8 Is Upregulated in the Tissue-Derived EVs of Odontogenic Keratocysts.

BioMed research international

Liu JF,Zhang CX,Li RF,Man QW

Wed Aug 10 00:00:00 EDT 2022

miR-596-3p suppresses brain metastasis of non-small cell lung cancer by modulating YAP1 and IL-8.

Cell death & disease

Li C,Zheng H,Xiong J,Huang Y,Li H,Jin H,Ai S,Wang Y,Su T,Sun G,Xiao X,Fu T,Wang Y,Gao X,Liang P

Fri Aug 12 00:00:00 EDT 2022

Endometrial stromal cell ferroptosis promotes angiogenesis in endometriosis.

Cell death discovery

Li G,Lin Y,Zhang Y,Gu N,Yang B,Shan S,Liu N,Ouyang J,Yang Y,Sun F,Xu H

Mon Jan 17 00:00:00 EST 2022

Endometrial stromal cell ferroptosis promotes angiogenesis in endometriosis.

Cell death discovery

Li G,Lin Y,Zhang Y,Gu N,Yang B,Shan S,Liu N,Ouyang J,Yang Y,Sun F,Xu H

Mon Jan 17 00:00:00 EST 2022

Endometrial stromal cell ferroptosis promotes angiogenesis in endometriosis.

Cell death discovery

Li G,Lin Y,Zhang Y,Gu N,Yang B,Shan S,Liu N,Ouyang J,Yang Y,Sun F,Xu H

Mon Jan 17 00:00:00 EST 2022

The role of PKM2 nuclear translocation in the constant activation of the NF-κB signaling pathway in cancer-associated fibroblasts.

Cell death & disease

Gu J,Li X,Zhao L,Yang Y,Xue C,Gao Y,Li J,Han Q,Sun Z,Bai C,Zhao RC

Wed Mar 17 00:00:00 EDT 2021

RIPK3 activation induces TRIM28 derepression in cancer cells and enhances the anti-tumor microenvironment.

Molecular cancer

Park HH,Kim HR,Park SY,Hwang SM,Hong SM,Park S,Kang HC,Morgan MJ,Cha JH,Lee D,Roe JS,Kim YS

Sat Aug 21 00:00:00 EDT 2021

LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.

EMBO reports

Sun X,Thorne RF,Zhang XD,He M,Li J,Feng S,Liu X,Wu M

Fri Apr 03 00:00:00 EDT 2020

MicroRNA-675-3p regulates IL-1β-stimulated human chondrocyte apoptosis and cartilage degradation by targeting GNG5.

Biochemical and biophysical research communications

Shen XF,Cheng Y,Dong QR,Zheng MQ

Thu Jun 25 00:00:00 EDT 2020

Interaction with tumor‑associated macrophages promotes PRL‑3‑induced invasion of colorectal cancer cells via MAPK pathway‑induced EMT and NF‑κB signaling‑induced angiogenesis.

Oncology reports

Zhang T,Liu L,Lai W,Zeng Y,Xu H,Lan Q,Su P,Chu Z

Wed May 01 00:00:00 EDT 2019

Necroptosis promotes cell-autonomous activation of proinflammatory cytokine gene expression.

Cell death & disease

Zhu K,Liang W,Ma Z,Xu D,Cao S,Lu X,Liu N,Shan B,Qian L,Yuan J

Tue May 01 00:00:00 EDT 2018

Radiotherapy induces an immediate inflammatory reaction in malignant glioma: a clinical microdialysis study.

Journal of neuro-oncology

Tabatabaei P,Visse E,Bergström P,Brännström T,Siesjö P,Bergenheim AT

Sun Jan 01 00:00:00 EST 2017

Expression, purification, and characterization of rhTyrRS.

BMC biotechnology

Lang Y,Zhang Y,Zhan L,Feng Z,Zhou X,Yu M,Mo W

Tue Jul 15 00:00:00 EDT 2014

Molecular crosstalk between cancer cells and tumor microenvironment components suggests potential targets for new therapeutic approaches in mobile tongue cancer.

Cancer medicine

Dayan D,Salo T,Salo S,Nyberg P,Nurmenniemi S,Costea DE,Vered M

Mon Oct 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: alveolar macrophage chemotactic factor I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; Granulocyte chemotactic protein 1; IL-8; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; MDNCF; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAP-1; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; RP11-537A6.8; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

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Gene Aliases: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

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UniProt ID: (Human) P10145

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Entrez Gene ID: (Human) 3576

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Function(s)

interleukin-8 receptor binding protein binding chemokine activity chemokine

Process(es)

angiogenesis response to molecule of bacterial origin movement of cell or subcellular component chemotaxis inflammatory response immune response cell cycle arrest signal transduction G-protein coupled receptor signaling pathway negative regulation of cell proliferation calcium-mediated signaling regulation of cell adhesion neutrophil chemotaxis receptor internalization response to endoplasmic reticulum stress intracellular signal transduction PERK-mediated unfolded protein response neutrophil activation cellular response to fibroblast growth factor stimulus regulation of single stranded viral RNA replication via double stranded DNA intermediate negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis embryonic digestive tract development induction of positive chemotaxis chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of neutrophil chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CXCL8/IL-8 Polyclonal Antibody

CXCL8/IL-8 Antibody (17038-1-AP) in IHC (P)

Product Details

17038-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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