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CXCL9 Antibody (500-P50-1MG) in ELISA
Sandwich ELISA: To detect Human MIG (CXCL9) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CXCL9 Polyclonal Antibody (Product # 500-P50-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CXCL9 Polyclonal Antibody, Biotin (Product # 500-P50BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIG (CXCL9).
Product Details
500-P50-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MIG (CXCL9)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930594
Product Specific Information
AA Sequence of recombinant protein: TPVVRKGRCS CISTNQGTIH LQSLKDLKQF APSPSCEKIE IIATLKNGVQ TCLNPDSADV KELIKKWEKQ VSQKKKQKNG KKHQKKKVLK VRKSQRSRQK KTT.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIG (CXCL9). Anti-Human MIG (CXCL9)-specific antibody was purified by affinity chromatography employing an immobilized Human MIG (CXCL9) matrix.
Sandwich ELISA: To detect Human MIG (CXCL9) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIG (CXCL9) (500-P50Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIG (CXCL9).
Western Blot: To detect Human MIG (CXCL9) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIG (CXCL9) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
MIG, also known as CXCL9, is a T-cell chemoattractant inducible by gamma interferon that is a member of the CXC chemokine family of cytokines.This gene is mapped to 4q21. It is noted that, although the best-described activities of the chemokines are as chemotactic factors, chemokines also have an effect on T-cell activation, angiogenesis, and HIV infection. While most CXC chemokines are chemotactic for neutrophils, MIG and INP10 are unusual and similar in being CXC chemokines that are chemotactic for lymphocytes and inactive in neutrophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 9; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 9; CXC; CXC motif chemokine; CXCL; Gamma-interferon-induced monokine; HuMIG; monokine induced by gamma interferon; Monokine induced by interferon-gamma; Small-inducible cytokine B9
Gene Aliases: CMK; crg-10; CXCL9; Humig; MIG; SCYB9
UniProt ID: (Human) Q07325
Entrez Gene ID: (Human) 4283
positive regulation of leukocyte chemotaxis
chemotaxis
defense response
inflammatory response
immune response
cellular defense response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
positive regulation of cAMP metabolic process
response to lipopolysaccharide
regulation of cell proliferation
positive regulation of cAMP-mediated signaling
positive regulation of myoblast differentiation
positive regulation of release of sequestered calcium ion into cytosol
defense response to virus
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of myoblast fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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