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Invitrogen
CXCL9 Recombinant Rabbit Monoclonal Antibody (11H1L14)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
CXCL9 Antibody (701117) in ICC/IF
Immunofluorescence analysis of CXCL9/MIG was performed using 70% confluent log phase THP-1 cells treated with 100 ng of IFN-gamma for 15 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CXCL9/MIG (11H1L14) Recombinant Rabbit Monoclonal Antibody (Product # 701117) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Cha... View More
Product Details
701117
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
11H1L14
Immunogen
Recombinant protein corresponding to amino acids 23-125 of human monokine induced by Interferon-gamma
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532396
Product Specific Information
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
MIG, also known as CXCL9, is a T-cell chemoattractant inducible by gamma interferon that is a member of the CXC chemokine family of cytokines.This gene is mapped to 4q21. It is noted that, although the best-described activities of the chemokines are as chemotactic factors, chemokines also have an effect on T-cell activation, angiogenesis, and HIV infection. While most CXC chemokines are chemotactic for neutrophils, MIG and INP10 are unusual and similar in being CXC chemokines that are chemotactic for lymphocytes and inactive in neutrophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 9; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 9; CXC; CXC motif chemokine; CXCL; gamma interferon-induced monokine; Gamma-interferon-induced monokine; HuMIG; M119; MIG; monokine induced by gamma interferon; Monokine induced by interferon-gamma; Protein m119; small inducible cytokine B subfamily (Cys-X-Cys), member 9; Small-inducible cytokine B9
Gene Aliases: BB139920; CMK; crg-10; CXCL9; Humig; MIG; MuMIG; SCYB9
UniProt ID: (Human) Q07325, (Mouse) P18340
Entrez Gene ID: (Human) 4283, (Mouse) 17329
positive regulation of leukocyte chemotaxis
chemotaxis
defense response
inflammatory response
immune response
cellular defense response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
positive regulation of cAMP metabolic process
response to lipopolysaccharide
regulation of cell proliferation
positive regulation of cAMP-mediated signaling
positive regulation of myoblast differentiation
positive regulation of release of sequestered calcium ion into cytosol
defense response to virus
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of myoblast fusion
T cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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