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Invitrogen
CXCL9 (MIG) Monoclonal Antibody (MIG-2F5.5), eFluor™ 660, eBioscience™
FIGURE: 1 / 3
CXCL9 (MIG) Antibody (50-3009-80) in Flow
RAW 264-7 cells were unstimulated (left) or stimulated with Mouse IFN gamma Recombinant Protein (Product # 14-8311-63) in the presence of Brefeldin A for 16 hours. Cells were intracellularly stained with Anti-Mouse CXCL9 (MIG) eFluor® 660 using the IC Fixation & Permeabilization Buffers (Product # 88-8823-88) and protocol. Total viable cells were used for analysis.
Product Details
50-3009-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MIG-2F5.5
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11218694
Product Specific Information
Description: The monoclonal antibody MIG-2F5.5 reacts with mouse monokine induced by IFN gamma (mig, mig-1, CXCL9). This 14.4 kDa inflammatory chemokine is specifically induced by IFN gamma, but not other activators such as LPS or IFN alpha. Secretion of mig, mainly by macrophages results in the chemotaxis of a variety of activated T cells via the CXCR3 chemokine receptor. Mig is involved many areas of research including autoimmune diseases, cancer, and inflammation.
Applications Reported: This MIG-2F5.5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This MIG-2F5.5 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated RAW 264.7 cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MIG, also known as CXCL9, is a T-cell chemoattractant inducible by gamma interferon that is a member of the CXC chemokine family of cytokines.This gene is mapped to 4q21. It is noted that, although the best-described activities of the chemokines are as chemotactic factors, chemokines also have an effect on T-cell activation, angiogenesis, and HIV infection. While most CXC chemokines are chemotactic for neutrophils, MIG and INP10 are unusual and similar in being CXC chemokines that are chemotactic for lymphocytes and inactive in neutrophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 9; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; gamma interferon-induced monokine; Gamma-interferon-induced monokine; M119; MIG; monokine induced by gamma interferon; Monokine induced by interferon-gamma; Protein m119; small inducible cytokine B subfamily (Cys-X-Cys), member 9; Small-inducible cytokine B9
Gene Aliases: BB139920; CMK; crg-10; Cxcl9; Mig; MuMIG; Scyb9
UniProt ID: (Mouse) P18340
Entrez Gene ID: (Mouse) 17329
positive regulation of leukocyte chemotaxis
chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
T cell chemotaxis
response to lipopolysaccharide
regulation of cell proliferation
positive regulation of myoblast differentiation
defense response to virus
chemokine-mediated signaling pathway
positive regulation of myoblast fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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