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Invitrogen
CXCR3 Recombinant Rabbit Monoclonal Antibody (6H1L8)
FIGURE: 1 / 2
CXCR3 Antibody (702228) in ICC/IF
For immunofluorescence analysis, K562 cells were fixed and permeabilized for detection of endogenous CXCR3 using Anti- CXCR3 Recombinant Rabbit Monoclonal Antibody (Product # 702228, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CXCR3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S3693... View More
Product Details
702228
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H1L8
Immunogen
Peptides corresponding to Human CXCR3 (aa 21-35, 352-368)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633069
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Goat and Cat
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to this protein. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. This gene is prominently expressed in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that this gene and its chemokines participate in the recruitment of inflammatory cells. Therefore, this protein is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: an; C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 3; CC motif chemokine; CCmotif chemokine; CD183; chemokine (C-X-C motif) receptor 3; chemokine receptor 3; CKR-L2; CXC; CXC motif chemokine; CXC-R3; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; IP10; IP10 receptor; Mig receptor
Gene Aliases: CD182; CD183; CKR-L2; CMKAR3; CXCR3; GPR9; IP10-R; Mig-R; MigR
UniProt ID: (Human) P49682
Entrez Gene ID: (Human) 2833
angiogenesis
regulation of leukocyte migration
apoptotic process
movement of cell or subcellular component
chemotaxis
inflammatory response
cell adhesion
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
T cell chemotaxis
calcium-mediated signaling
chemokine-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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