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Invitrogen
CXCR4 Recombinant Rabbit Monoclonal Antibody (21H17L11)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
CXCR4 Antibody (704015) in ICC/IF
HEK-293 cells stably transfected with CXCR4 were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with CXCR4 Recombinant Rabbit Monoclonal Antibody (Product # 704015, 1:500 dilution). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mount... View More
Product Details
704015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
21H17L11
Immunogen
Peptides corresponding to residues 338-359 of Human CXCR4 receptor
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2895898
Target Information
CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C) receptor 4; chemokine receptor; chemokine receptor 4; CXC; CXC motif chemokine; CXC-R4; CXCR-4; FB22; Fusin; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; PB-CKR; Pre-B-cell-derived chemokine receptor; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor
Gene Aliases: b2b220Clo; CD184; Cmkar4; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; PB-CKR; PBSF/SDF-1; Sdf1r; WHIM; WHIMS
UniProt ID: (Human) P61073, (Mouse) P70658
Entrez Gene ID: (Human) 7852, (Mouse) 12767
virus receptor activity
actin binding
G-protein coupled receptor activity
protein binding
drug binding
coreceptor activity
C-X-C chemokine receptor activity
cytokine binding
ubiquitin protein ligase binding
myosin light chain binding
ubiquitin binding
signal transducer activity
chemokine receptor activity
patterning of blood vessels
ameboidal-type cell migration
neuron migration
epithelial cell development
chemotaxis
signal transduction
G-protein coupled receptor signaling pathway
germ cell development
nervous system development
brain development
motor neuron axon guidance
germ cell migration
organ morphogenesis
cell migration
calcium-mediated signaling
telencephalon cell migration
regulation of cell migration
T cell proliferation
neutrophil activation
regulation of programmed cell death
myelin maintenance
positive regulation of oligodendrocyte differentiation
regulation of chemotaxis
regulation of calcium ion transport
cardiac muscle contraction
chemokine-mediated signaling pathway
cellular response to cytokine stimulus
activation of MAPK activity
response to hypoxia
dendritic cell chemotaxis
apoptotic process
inflammatory response
positive regulation of cytosolic calcium ion concentration
neuron recognition
response to virus
fusion of virus membrane with host plasma membrane
entry into host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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