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CYP17A1 Polyclonal Antibody

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Antibody Testing Data (11)

CYP17A1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

CYP17A1 Antibody (14447-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using CYP17A1 antibody 14447-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

CYP17A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

14447-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:20-1:200

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CYP17A1 Fusion Protein Ag5899 (160-508 aa encoded by BC062997)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: HNGQSIDIS FPVFVAVTNV ISLICFNTSY KNGDPELNVI QNYNEGIIDN LSKDSLVDLV PWLKIFPNKT LEKLKSHVKI RNDLLNKILE NYKEKFRSDS ITNMLDTLMQ AKMNSDNGNA GPDQDSELLS DNHILTTIGD IFGAGVETTT SVVKWTLAFL LHNPQVKKKL YEEIDQNVGF SRTPTISDRN RLLLLEATIR EVLRLRPVAP MLIPHKANVD SSIGEFAVDK GTEVIINLWA LHHNEKEWHQ PDQFMPERFL NPAGTQLISP SVSYLPFGAG PRSCIGEILA RQELFLIMAW LLQRFDLEVP DDGQLPSLEG IPKVVFLIDS FKVKIKVRQA WREAQAEGST (160-508 aa encoded by BC062997)

Target Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Involvement of p38 MAPK in Leydig cell aging and age-related decline in testosterone.

Frontiers in endocrinology

Luo D,Qi X,Xu X,Yang L,Yu C,Guan Q

Fri Mar 24 00:00:00 EDT 2023

Involvement of p38 MAPK in Leydig cell aging and age-related decline in testosterone.

Frontiers in endocrinology

Luo D,Qi X,Xu X,Yang L,Yu C,Guan Q

Fri Mar 24 00:00:00 EDT 2023

Persistence of foetal testicular features in patients with defective androgen signalling.

European journal of endocrinology

Al-Sharkawi M,Calonga-Solís V,Dressler FF,Busch H,Hiort O,Werner R

Tue Jan 10 00:00:00 EST 2023

Bisphenol S induces oxidative stress-mediated impairment of testosterone synthesis by inhibiting the Nrf2/HO-1 signaling pathway.

Journal of biochemical and molecular toxicology

Wang YX,Dai W,Li YZ,Wu ZY,Kan YQ,Zeng HC,He QZ

Wed Mar 01 00:00:00 EST 2023

Luteinizing hormone receptor promotes angiogenesis in ovarian endothelial cells of Macaca fascicularis and Homo sapiens†.

Biology of reproduction

Lund M,Pearson AC,Sage MAG,Duffy DM

Mon Feb 13 00:00:00 EST 2023

The molecular mechanism of miR-96-5p in the pathogenesis and treatment of polycystic ovary syndrome.

Translational research : the journal of laboratory and clinical medicine

Liu Y,Zhang S,Chen L,Huang X,Wang M,Ponikwicka-Tyszko D,Rahman NA,Wolczynski S,Yao B,Li X

Thu Jun 01 00:00:00 EDT 2023

Rat Ovarian Function Is Impaired during Experimental Autoimmune Encephalomyelitis.

Cells

Milosevic A,Lavrnja I,Savic D,Milosevic K,Skuljec J,Bjelobaba I,Janjic MM

Thu Mar 30 00:00:00 EDT 2023

Low-Dose Sodium Salicylate Promotes Ovulation by Regulating Steroids via CYP17A1.

International journal of molecular sciences

Li T,Ren X,Li T,Yu L,Teng M,Zheng Y,Lei A

Mon Jan 30 00:00:00 EST 2023

Low-Dose Sodium Salicylate Promotes Ovulation by Regulating Steroids via CYP17A1.

International journal of molecular sciences

Li T,Ren X,Li T,Yu L,Teng M,Zheng Y,Lei A

Mon Jan 30 00:00:00 EST 2023

Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.

Particle and fibre toxicology

Jin H,Yan M,Pan C,Liu Z,Sha X,Jiang C,Li L,Pan M,Li D,Han X,Ding J

Thu Feb 17 00:00:00 EST 2022

Astragalus polysaccharide protects sepsis model rats after cecum ligation and puncture.

Frontiers in bioengineering and biotechnology

Li J,Zhao J,Chai Y,Li W,Liu X,Chen Y

Tue Nov 08 00:00:00 EST 2022

Astragalus polysaccharide protects sepsis model rats after cecum ligation and puncture.

Frontiers in bioengineering and biotechnology

Li J,Zhao J,Chai Y,Li W,Liu X,Chen Y

Tue Nov 08 00:00:00 EST 2022

Excess dietary zinc drives a Cushing's-like syndrome in ovariectomized mice - Implications for postmenopausal obesity.

Biochemical and biophysical research communications

Zhao Y,Wang Y,Hu L,Chen H,Xiao X,Li Z,Feng M,Yang P,Liu P,Zhang S,Xiao G,Liu J,Shen J,Bai X

Sat Nov 19 00:00:00 EST 2022

Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells.

The Journal of steroid biochemistry and molecular biology

Wang Q,Yan Q,Nan J,Wang J,Zhang Y,Zhao X

Sat Oct 01 00:00:00 EDT 2022

Effect of iodoacetic acid on the reproductive system of male mice.

Frontiers in pharmacology

Liang Y,Huang X,Fang L,Wang M,Yu C,Guan Q

Tue Sep 13 00:00:00 EDT 2022

Effect of iodoacetic acid on the reproductive system of male mice.

Frontiers in pharmacology

Liang Y,Huang X,Fang L,Wang M,Yu C,Guan Q

Tue Sep 13 00:00:00 EDT 2022

A Novel SEMA3G Mutation in Two Siblings Affected by Syndromic GnRH Deficiency.

Neuroendocrinology

Oleari R,André V,Lettieri A,Tahir S,Roth L,Paganoni A,Eberini I,Parravicini C,Scagliotti V,Cotellessa L,Bedogni F,De Martini LB,Corridori MV,Gulli S,Augustin HG,Gaston-Massuet C,Hussain K,Cariboni A

Wed Nov 17 00:00:00 EST 2021

Effects of increasing intake of soybean oil on synthesis of testosterone in Leydig cells.

Nutrition & metabolism

Su Y,Tian Z,Qi X,Luo D,Liu L,Liu S,Zheng D,Wei F,He Z,Guan Q

Wed May 26 00:00:00 EDT 2021

WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility.

Development (Cambridge, England)

Habara O,Logan CY,Kanai-Azuma M,Nusse R,Takase HM

Sat May 01 00:00:00 EDT 2021

Effect of the spatial-temporal specific theca cell Cyp17 overexpression on the reproductive phenotype of the novel TC17 mouse.

Journal of translational medicine

Secchi C,Belli M,Harrison TNH,Swift J,Ko C,Duleba AJ,Stupack D,Chang RJ,Shimasaki S

Fri Oct 15 00:00:00 EDT 2021

Expression and localisation of ephrin-B1 and EphB4 in steroidogenic cells in the naturally cycling mouse ovary.

Reproductive biology

Alam J,Ogawa K

Wed Sep 01 00:00:00 EDT 2021

3-Chloro-1, 2-propanediol inhibits autophagic flux by impairment of lysosomal function in HepG2 cells.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Lu J,Lu J,Chen Y,Feng Z,Liu S,Guan S

Thu Oct 01 00:00:00 EDT 2020

Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance, mitochondrial dysfunction and oxidative stress.

Biological research

Peng Y,Yang X,Luo X,Liu C,Cao X,Wang H,Guo L

Tue Oct 27 00:00:00 EDT 2020

Expression and localisation of ephrin-B1, EphB2, and EphB4 in the mouse testis during postnatal development.

Reproductive biology

Gofur MR,Alam J,Ogawa K

Tue Sep 01 00:00:00 EDT 2020

Features of the fetal gonad in androgen synthesis in the postpubertal testis are preserved in complete androgen insensitivity syndrome due to a novel genetic splice site donor variant in androgen receptor gene intron 1.

The Journal of steroid biochemistry and molecular biology

Jarzabek K,Koda M,Chrusciel M,Kanczuga-Koda L,Sobczynska-Tomaszewska A,Rahman NA,Wolczynski S

Tue Oct 01 00:00:00 EDT 2019

Exposure to cadmium during gestation and lactation affects development and function of Leydig cells in male offspring.

Environmental toxicology

Tian H,Chen S,Leng Y,Li T,Li Z,Chen H,Zhang Q

Thu Mar 01 00:00:00 EST 2018

Exposure to cadmium during gestation and lactation affects development and function of Leydig cells in male offspring.

Environmental toxicology

Tian H,Chen S,Leng Y,Li T,Li Z,Chen H,Zhang Q

Thu Mar 01 00:00:00 EST 2018

Exposure to cadmium during gestation and lactation affects development and function of Leydig cells in male offspring.

Environmental toxicology

Tian H,Chen S,Leng Y,Li T,Li Z,Chen H,Zhang Q

Thu Mar 01 00:00:00 EST 2018

Exposure to cadmium during gestation and lactation affects development and function of Leydig cells in male offspring.

Environmental toxicology

Tian H,Chen S,Leng Y,Li T,Li Z,Chen H,Zhang Q

Thu Mar 01 00:00:00 EST 2018

The Involvement of Granulosa Cells in the Regulation by Gonadotropins of Cyp17a1 in Theca Cells.

In vivo (Athens, Greece)

Kakuta H,Iguchi T,Sato T

Mon Jan 28 00:00:00 EST 2019

The Involvement of Granulosa Cells in the Regulation by Gonadotropins of Cyp17a1 in Theca Cells.

In vivo (Athens, Greece)

Kakuta H,Iguchi T,Sato T

Mon Jan 28 00:00:00 EST 2019

Deleting the mouse Hsd17b1 gene results in a hypomorphic Naglu allele and a phenotype mimicking a lysosomal storage disease.

Scientific reports

Jokela H,Hakkarainen J,Kätkänaho L,Pakarinen P,Ruohonen ST,Tena-Sempere M,Zhang FP,Poutanen M

Mon Nov 27 00:00:00 EST 2017

Deleting the mouse Hsd17b1 gene results in a hypomorphic Naglu allele and a phenotype mimicking a lysosomal storage disease.

Scientific reports

Jokela H,Hakkarainen J,Kätkänaho L,Pakarinen P,Ruohonen ST,Tena-Sempere M,Zhang FP,Poutanen M

Mon Nov 27 00:00:00 EST 2017

Transcriptome Analysis of WHV/c-myc Transgenic Mice Implicates Cytochrome P450 Enzyme 17A1 as a Promising Biomarker for Hepatocellular Carcinoma.

Cancer prevention research (Philadelphia, Pa.)

Wang F,Huang J,Zhu Z,Ma X,Cao L,Zhang Y,Chen W,Dong Y

Thu Sep 01 00:00:00 EDT 2016

Transcriptome Analysis of WHV/c-myc Transgenic Mice Implicates Cytochrome P450 Enzyme 17A1 as a Promising Biomarker for Hepatocellular Carcinoma.

Cancer prevention research (Philadelphia, Pa.)

Wang F,Huang J,Zhu Z,Ma X,Cao L,Zhang Y,Chen W,Dong Y

Thu Sep 01 00:00:00 EDT 2016

Hydroxysteroid (17β)-dehydrogenase 1-deficient female mice present with normal puberty onset but are severely subfertile due to a defect in luteinization and progesterone production.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hakkarainen J,Jokela H,Pakarinen P,Heikelä H,Kätkänaho L,Vandenput L,Ohlsson C,Zhang FP,Poutanen M

Tue Sep 01 00:00:00 EDT 2015

Hydroxysteroid (17β)-dehydrogenase 1-deficient female mice present with normal puberty onset but are severely subfertile due to a defect in luteinization and progesterone production.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hakkarainen J,Jokela H,Pakarinen P,Heikelä H,Kätkänaho L,Vandenput L,Ohlsson C,Zhang FP,Poutanen M

Tue Sep 01 00:00:00 EDT 2015

Antiandrogenic mechanisms of pesticides in human LNCaP prostate and H295R adrenocortical carcinoma cells.

Toxicological sciences : an official journal of the Society of Toxicology

Robitaille CN,Rivest P,Sanderson JT

Thu Jan 01 00:00:00 EST 2015

Antiandrogenic mechanisms of pesticides in human LNCaP prostate and H295R adrenocortical carcinoma cells.

Toxicological sciences : an official journal of the Society of Toxicology

Robitaille CN,Rivest P,Sanderson JT

Thu Jan 01 00:00:00 EST 2015

Intra-tissue steroid profiling indicates differential progesterone and testosterone metabolism in the endometrium and endometriosis lesions.

The Journal of clinical endocrinology and metabolism

Huhtinen K,Saloniemi-Heinonen T,Keski-Rahkonen P,Desai R,Laajala D,Ståhle M,Häkkinen MR,Awosanya M,Suvitie P,Kujari H,Aittokallio T,Handelsman DJ,Auriola S,Perheentupa A,Poutanen M

Sat Nov 01 00:00:00 EDT 2014

Intra-tissue steroid profiling indicates differential progesterone and testosterone metabolism in the endometrium and endometriosis lesions.

The Journal of clinical endocrinology and metabolism

Huhtinen K,Saloniemi-Heinonen T,Keski-Rahkonen P,Desai R,Laajala D,Ståhle M,Häkkinen MR,Awosanya M,Suvitie P,Kujari H,Aittokallio T,Handelsman DJ,Auriola S,Perheentupa A,Poutanen M

Sat Nov 01 00:00:00 EDT 2014

Increased expression of CYP17A1 indicates an effective targeting of the androgen receptor axis in castration resistant prostate cancer (CRPC).

SpringerPlus

Bremmer F,Jarry H,Strauß A,Behnes CL,Trojan L,Thelen P

Tue Oct 21 00:00:00 EDT 2014

Increased expression of CYP17A1 indicates an effective targeting of the androgen receptor axis in castration resistant prostate cancer (CRPC).

SpringerPlus

Bremmer F,Jarry H,Strauß A,Behnes CL,Trojan L,Thelen P

Tue Oct 21 00:00:00 EDT 2014

Ectopic cortisol-producing adrenocortical adenoma in the renal hilum: histopathological features and steroidogenic enzyme profile.

International journal of clinical and experimental pathology

Tong A,Jia A,Yan S,Zhang Y,Xie Y,Liu G

Thu May 14 00:00:00 EDT 2015

Ectopic cortisol-producing adrenocortical adenoma in the renal hilum: histopathological features and steroidogenic enzyme profile.

International journal of clinical and experimental pathology

Tong A,Jia A,Yan S,Zhang Y,Xie Y,Liu G

Thu May 14 00:00:00 EDT 2015

Transgenic GATA-4 expression induces adrenocortical tumorigenesis in C57Bl/6 mice.

Journal of cell science

Chrusciel M,Vuorenoja S,Mohanty B,Rivero-Müller A,Li X,Toppari J,Huhtaniemi I,Rahman NA

Mon Apr 15 00:00:00 EDT 2013

Transgenic GATA-4 expression induces adrenocortical tumorigenesis in C57Bl/6 mice.

Journal of cell science

Chrusciel M,Vuorenoja S,Mohanty B,Rivero-Müller A,Li X,Toppari J,Huhtaniemi I,Rahman NA

Mon Apr 15 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 17-alpha-hydroxyprogesterone aldolase; 17a-hydroxylase; CYPXVII; Cytochrome P450 17A1; cytochrome p450 XVIIA1; cytochrome P450, 17; cytochrome P450, 17a1; cytochrome P450, family 17, subfamily A, polypeptide 1; cytochrome P450, subfamily 17; Cytochrome P450, subfamily XVII; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia; Cytochrome P450-C17; Cytochrome P450c17; HGNC:2593; P450-C17; P45017alpha; P450c17; steroid 17-alpha hydroxylase; Steroid 17-alpha-hydroxylase/17,20 lyase; Steroid 17-alpha-monooxygenase

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Gene Aliases: CPT7; CYP17; CYP17A1; P450C17; S17AH

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UniProt ID: (Human) P05093, (Mouse) P27786, (Rat) P11715

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Entrez Gene ID: (Human) 1586, (Mouse) 13074, (Rat) 25146

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Function(s)

steroid 17-alpha-monooxygenase activity iron ion binding oxygen binding heme binding 17-alpha-hydroxyprogesterone aldolase activity monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen lyase activity metal ion binding

Process(es)

steroid biosynthetic process glucocorticoid biosynthetic process steroid metabolic process hormone biosynthetic process progesterone metabolic process oxidation-reduction process positive regulation of steroid hormone biosynthetic process androgen biosynthetic process sex differentiation response to herbicide response to acetate response to ionizing radiation sterol metabolic process response to insecticide biphenyl metabolic process dibenzo-p-dioxin metabolic process phenol-containing compound metabolic process phthalate metabolic process hippocampus development adrenal gland development ovulation response to nutrient levels response to retinoic acid Leydig cell differentiation response to cytokine response to drug response to steroid hormone response to cAMP response to methylmercury response to fungicide cellular response to lipopolysaccharide cellular response to antibiotic cellular response to gonadotropin stimulus organic acid metabolic process male gonad development response to toxic substance response to organic substance response to organic cyclic compound response to gonadotropin

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CYP17A1 Polyclonal Antibody

CYP17A1 Antibody (14447-1-AP) in ICC/IF

Product Details

14447-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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