Proteintech

CYP1A2 Polyclonal Antibody

CYP1A2 Antibody in Immunocytochemistry (ICC/IF)

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CYP1A2 Antibody (19936-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CYP1A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CYP1A2 Antibody in Flow Cytometry (Flow)

CYP1A2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

19936-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 23 publications 23 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to CYP1A2.

Target Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Coptisine modulates the pharmacokinetics of florfenicol by targeting CYP1A2, CYP2C11 and CYP3A1 in the liver and P-gp in the jejunum of rats: a pilot study.

Xenobiotica; the fate of foreign compounds in biological systems

Li SC,Zhang M,Wang B,Li XT,Liang G

Wed Mar 01 00:00:00 EST 2023

Integration of proteomics and network toxicology reveals the mechanism of mercury chloride induced hepatotoxicity, in mice and HepG2 cells.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Cao X,Mao K,Zhang Y,Yang M,Liu H,Wang X,Hao L

Sat Jul 01 00:00:00 EDT 2023

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice.

Cell host & microbe

Zeng Y,Wu R,Wang F,Li S,Li L,Li Y,Qin P,Wei M,Yang J,Wu J,Chen A,Ke G,Yan Z,Yang H,Chen Z,Wang Z,Xiao W,Jiang Y,Chen X,Zeng Z,Zhao X,Chen P,Gong S

Wed May 10 00:00:00 EDT 2023

Microneedle array facilitates hepatic sinusoid construction in a large-scale liver-acinus-chip microsystem.

Microsystems & nanoengineering

Li S,Li C,Khan MI,Liu J,Shi Z,Gao D,Qiu B,Ding W

Thu Sep 28 00:00:00 EDT 2023

Humanized liver TK-NOG mice with functional deletion of hepatic murine cytochrome P450s as a model for studying human drug metabolism.

Scientific reports

Uehara S,Iida Y,Ida-Tanaka M,Goto M,Kawai K,Yamamoto M,Higuchi Y,Ito S,Takahashi R,Kamimura H,Ito M,Yamazaki H,Oshimura M,Kazuki Y,Suemizu H

Thu Sep 01 00:00:00 EDT 2022

Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice.

British journal of pharmacology

Jiang LP,Ji JZ,Ge PX,Zhu T,Mi QY,Tai T,Li YF,Xie HG

Sat Jan 01 00:00:00 EST 2022

Kinetic Characteristics of Curcumin and Germacrone in Rat and Human Liver Microsomes: Involvement of CYP Enzymes.

Molecules (Basel, Switzerland)

Su S,Wu H,Zhou J,Yuan G,Wang H,Feng J

Thu Jul 14 00:00:00 EDT 2022

Intermittent Hypoxia Inhibits Hepatic CYP1a2 Expression and Delays Aminophylline Metabolism.

Evidence-based complementary and alternative medicine : eCAM

Zhang XB,Chen XY,Chiu KY,He XZ,Wang JM,Zeng HQ,Zeng Y

Sat Jul 16 00:00:00 EDT 2022

Gut microbiota and host Cyp450s co-contribute to pharmacokinetic variability in mice with non-alcoholic steatohepatitis: Effects vary from drug to drug.

Journal of advanced research

Guo J,Xu Y,Chen LJ,Zhang SX,Liou YL,Chen XP,Tan ZR,Zhou HH,Zhang W,Chen Y

Fri Jul 01 00:00:00 EDT 2022

Proteomic and Bioinformatics Analysis of Membrane Lipid Domains after Brij 98 Solubilization of Uninduced and Phenobarbital-Induced Rat Liver Microsomes: Defining the Membrane Localization of the P450 Enzyme System.

Drug metabolism and disposition: the biological fate of chemicals

Reed JR,Guidry JJ,Backes WL

Fri Apr 01 00:00:00 EDT 2022

Aryl Hydrocarbon Receptor Activity in Hepatocytes Sensitizes to Hyperacute Acetaminophen-Induced Hepatotoxicity in Mice.

Cellular and molecular gastroenterology and hepatology

Schuran FA,Lommetz C,Steudter A,Ghallab A,Wieschendorf B,Schwinge D,Zuehlke S,Reinders J,Heeren J,Lohse AW,Schramm C,Herkel J,Carambia A

Mon Dec 20 00:00:00 EST 2021

Aryl Hydrocarbon Receptor Activity in Hepatocytes Sensitizes to Hyperacute Acetaminophen-Induced Hepatotoxicity in Mice.

Cellular and molecular gastroenterology and hepatology

Schuran FA,Lommetz C,Steudter A,Ghallab A,Wieschendorf B,Schwinge D,Zuehlke S,Reinders J,Heeren J,Lohse AW,Schramm C,Herkel J,Carambia A

Mon Dec 20 00:00:00 EST 2021

Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.

Acta biomaterialia

Du K,Li S,Li C,Li P,Miao C,Luo T,Qiu B,Ding W

Fri Oct 15 00:00:00 EDT 2021

Macrophage potentiates the recovery of liver zonation and metabolic function after acute liver injury.

Scientific reports

Miura A,Hosono T,Seki T

Thu May 06 00:00:00 EDT 2021

Macrophage potentiates the recovery of liver zonation and metabolic function after acute liver injury.

Scientific reports

Miura A,Hosono T,Seki T

Thu May 06 00:00:00 EDT 2021

Short-term standard alcohol consumption enhances platelet response to clopidogrel through inhibition of Nrf2/Ces1 pathway and induction of Cyp2c in mice.

Life sciences

Ge PX,Jiang LP,Tai T,Zhu T,Ji JZ,Li YF,Mi QY,Xie HG

Sun Aug 15 00:00:00 EDT 2021

Identification of the contact region responsible for the formation of the homomeric CYP1A2•CYP1A2 complex.

The Biochemical journal

Saha A,Connick JP,Reed JR,Lott CS,Backes WL

Fri Jun 11 00:00:00 EDT 2021

AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma.

Signal transduction and targeted therapy

Zhu Q,Ma Y,Liang J,Wei Z,Li M,Zhang Y,Liu M,He H,Qu C,Cai J,Wang X,Zeng Y,Jiao Y

Mon Aug 09 00:00:00 EDT 2021

Effects of Saikosaponin D on CYP1A2 and CYP2D6 in HepaRG Cells.

Drug design, development and therapy

Li H,Tang Y,Wang Y,Wei W,Yin C,Tang F

Mon Aug 23 00:00:00 EDT 2021

Sensing the Generation of Intracellular Free Electrons Using the Inactive Catalytic Subunit of Cytochrome P450s as a Sink.

Sensors (Basel, Switzerland)

Akintade DD,Chaudhuri B

Tue Jul 21 00:00:00 EDT 2020

Knockdown of Long Noncoding RNAs Hepatocyte Nuclear Factor 1α Antisense RNA 1 and Hepatocyte Nuclear Factor 4α Antisense RNA 1 Alters Susceptibility of Acetaminophen-Induced Cytotoxicity in HepaRG Cells.

Molecular pharmacology

Chen L,Wang P,Manautou JE,Zhong XB

Wed Apr 01 00:00:00 EDT 2020

CYP enzymes, expressed within live human suspension cells, are superior to widely-used microsomal enzymes in identifying potent CYP1A1/CYP1B1 inhibitors: Identification of quinazolinones as CYP1A1/CYP1B1 inhibitors that efficiently reverse B[a]P toxicity and cisplatin resistance.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Sonawane VR,Siddique MUM,Gatchie L,Williams IS,Bharate SB,Jayaprakash V,Sinha BN,Chaudhuri B

Mon Apr 01 00:00:00 EDT 2019

Drug resistance induces the upregulation of H₂S-producing enzymes in HCT116 colon cancer cells.

Biochemical pharmacology

Untereiner AA,Pavlidou A,Druzhyna N,Papapetropoulos A,Hellmich MR,Szabo C

Thu Mar 01 00:00:00 EST 2018

Drug resistance induces the upregulation of H₂S-producing enzymes in HCT116 colon cancer cells.

Biochemical pharmacology

Untereiner AA,Pavlidou A,Druzhyna N,Papapetropoulos A,Hellmich MR,Szabo C

Thu Mar 01 00:00:00 EST 2018

Decreased expression of hepatic cytochrome P450 1A2 (CYP1A2) in a chronic intermittent hypoxia mouse model.

Journal of thoracic disease

Zhang XB,Zeng YM,Chen XY,Zhang YX,Ding JZ,Xue C

Thu Feb 01 00:00:00 EST 2018

Decreased expression of hepatic cytochrome P450 1A2 (CYP1A2) in a chronic intermittent hypoxia mouse model.

Journal of thoracic disease

Zhang XB,Zeng YM,Chen XY,Zhang YX,Ding JZ,Xue C

Thu Feb 01 00:00:00 EST 2018

Schisantherin A alleviated alcohol-induced liver injury by the regulation of alcohol metabolism and NF-kB pathway.

Experimental animals

Li B,Li D,Wang Y,Meng X,Sun X,Tian J,Shi L,Ma F

Thu Nov 01 00:00:00 EDT 2018

Schisantherin A alleviated alcohol-induced liver injury by the regulation of alcohol metabolism and NF-kB pathway.

Experimental animals

Li B,Li D,Wang Y,Meng X,Sun X,Tian J,Shi L,Ma F

Thu Nov 01 00:00:00 EDT 2018

Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.

Cell death & disease

Wang C,Xu W,Zhang Y,Huang D,Huang K

Thu Jul 26 00:00:00 EDT 2018

Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.

Cell death & disease

Wang C,Xu W,Zhang Y,Huang D,Huang K

Thu Jul 26 00:00:00 EDT 2018

Hepatic, Metabolic, and Toxicity Evaluation of Repeated Oral Administration of SnS2 Nanoflowers in Mice.

Toxicological sciences : an official journal of the Society of Toxicology

Bai D,Li Q,Xiong Y,Wang C,Shen P,Bai L,Yuan L,Wu P

Wed Aug 01 00:00:00 EDT 2018

Hepatic, Metabolic, and Toxicity Evaluation of Repeated Oral Administration of SnS2 Nanoflowers in Mice.

Toxicological sciences : an official journal of the Society of Toxicology

Bai D,Li Q,Xiong Y,Wang C,Shen P,Bai L,Yuan L,Wu P

Wed Aug 01 00:00:00 EDT 2018

Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.

Scientific reports

Gao Y,Wang X,Sang Z,Li Z,Liu F,Mao J,Yan D,Zhao Y,Wang H,Li P,Ying X,Zhang X,He K,Wang H

Wed Apr 05 00:00:00 EDT 2017

Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.

Scientific reports

Gao Y,Wang X,Sang Z,Li Z,Liu F,Mao J,Yan D,Zhao Y,Wang H,Li P,Ying X,Zhang X,He K,Wang H

Wed Apr 05 00:00:00 EDT 2017

Self-renewal of hepatoblasts under chemically defined conditions by iterative growth factor and chemical screening.

Hepatology (Baltimore, Md.)

Lv L,Han Q,Chu Y,Zhang M,Sun L,Wei W,Jin C,Li W

Thu Jan 01 00:00:00 EST 2015

Self-renewal of hepatoblasts under chemically defined conditions by iterative growth factor and chemical screening.

Hepatology (Baltimore, Md.)

Lv L,Han Q,Chu Y,Zhang M,Sun L,Wei W,Jin C,Li W

Thu Jan 01 00:00:00 EST 2015

Different expression patterns of hepatic cytochrome P450 s during anaphylactic or lipopolysaccharide-induced inflammation.

Die Pharmazie

Moriya N,Kataoka H,Fujino H,Nishikawa J,Kugawa F

Sat Feb 01 00:00:00 EST 2014

Different expression patterns of hepatic cytochrome P450 s during anaphylactic or lipopolysaccharide-induced inflammation.

Die Pharmazie

Moriya N,Kataoka H,Fujino H,Nishikawa J,Kugawa F

Sat Feb 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: aromatic compound inducible; aryl hydrocarbon hydroxylase; Cholesterol 25-hydroxylase; CYP; CYPIA2; Cytochrome P(3)450; Cytochrome P-448; Cytochrome P-450d; Cytochrome P450 1A2; Cytochrome P450 4; cytochrome P450 family 1 subfamily a polypeptide 1; Cytochrome P450 subfamily I (aromatic compound-inducible) member A2 (Q42 form d); cytochrome P450, 1a2; cytochrome P450, 1a2, aromatic compound inducible; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound-inducible), member A2 (Q42, form d); cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; Cytochrome P450-D; Cytochrome P450-P2; Cytochrome P450-P3; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; Hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P(3)450; P-448; P450; P450 4; P450 form 4; P450-D; P450-P3; xenobiotic monooxygenase

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Gene Aliases: CP12; Cyp1a-2; Cyp1a1; CYP1A2; CYPD45; P-450d; P3-450; P450(PA); P450-3; RATCYPD45

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UniProt ID: (Human) P05177, (Mouse) P00186, (Rat) P04799

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Entrez Gene ID: (Human) 1544, (Mouse) 13077, (Rat) 24297

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Function(s)

monooxygenase activity iron ion binding electron carrier activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen oxygen binding enzyme binding heme binding demethylase activity caffeine oxidase activity aromatase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding oxidoreductase oxygenase metabolite interconversion enzyme

Process(es)

steroid catabolic process porphyrin-containing compound metabolic process xenobiotic metabolic process toxin biosynthetic process post-embryonic development alkaloid metabolic process regulation of gene expression monoterpenoid metabolic process drug metabolic process dibenzo-p-dioxin metabolic process epoxygenase P450 pathway lung development methylation response to estradiol response to lipopolysaccharide monocarboxylic acid metabolic process response to immobilization stress drug catabolic process exogenous drug catabolic process cellular respiration heterocycle metabolic process hydrogen peroxide biosynthetic process oxidation-reduction process oxidative demethylation cellular response to cadmium ion oxidative deethylation omega-hydroxylase P450 pathway cellular aromatic compound metabolic process toxin metabolic process response to organic substance response to organic cyclic compound response to drug

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CYP1A2 Polyclonal Antibody

CYP1A2 Antibody (19936-1-AP) in ICC/IF

Product Details

19936-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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