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CYP1B1 Antibody (PA5-119969) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat skeletal muscle tissue using CYP1B1 Polyclonal Antibody (Product # PA5-119969). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the CYP1B1 antibody at a dilutio... View More
Product Details
PA5-119969
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human CYP1B1 aa 231-400
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2913541
Product Specific Information
Positive Control: A549 cell lysate, Hela cell lysate, rat skeletal muscle tissue.
Subcellular Location: Mitochondrion, Endoplasmic reticulum membrane, Microsome membrane.
Target Information
CYP1B1 is a 543 amino acid intracellular protein localized to the endoplasmic reticulum. It belongs to the cytochrome P450 super family of enzymes which are monooxygenases and it catalyzes many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol and is also involved in the metabolism of substances active in the eye growth and differentiation and hence plays a putative role in ocular development. Mutated proteins are known to cause primary congenital glaucoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aryl hydrocarbon hydroxylase; CYPIB1; Cytochrome P450 1B1; cytochrome P450, family 1, subfamily B, polypeptide 1; cytochrome P450, subfamily 1B, polypeptide 1; cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile); Cytochrome P450RAP; flavoprotein-linked monooxygenase; Hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P450RAP; xenobiotic monooxygenase
Gene Aliases: CP1B; CYP1B1; CYPIB1; GLC3A; P4501B1
UniProt ID: (Human) Q16678, (Rat) Q64678
Entrez Gene ID: (Human) 1545, (Rat) 25426
monooxygenase activity
iron ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
heme binding
aromatase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase
oxygenase
metabolite interconversion enzyme
angiogenesis
trabecular meshwork development
cellular aromatic compound metabolic process
xenobiotic metabolic process
nitric oxide biosynthetic process
cell adhesion
visual perception
steroid metabolic process
estrogen metabolic process
negative regulation of cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
toxin metabolic process
response to toxic substance
positive regulation of vascular endothelial growth factor production
sterol metabolic process
arachidonic acid metabolic process
epoxygenase P450 pathway
collagen fibril organization
negative regulation of cell migration
negative regulation of NF-kappaB transcription factor activity
negative regulation of cell adhesion mediated by integrin
retinol metabolic process
retinal metabolic process
positive regulation of apoptotic process
endothelial cell migration
positive regulation of angiogenesis
positive regulation of JAK-STAT cascade
membrane lipid catabolic process
blood vessel morphogenesis
oxidation-reduction process
retinal blood vessel morphogenesis
cellular response to hydrogen peroxide
cellular response to organic cyclic compound
endothelial cell-cell adhesion
omega-hydroxylase P450 pathway
regulation of reactive oxygen species metabolic process
DNA modification
response to nutrient
male gonad development
response to organic substance
response to organic cyclic compound
positive regulation of smooth muscle cell migration
dibenzo-p-dioxin metabolic process
adrenal gland development
response to follicle-stimulating hormone
response to estradiol
benzene-containing compound metabolic process
estrous cycle
positive regulation of translation
response to arsenic-containing substance
response to steroid hormone
retina vasculature development in camera-type eye
ganglion development
cellular response to cAMP
cellular response to tumor necrosis factor
cellular response to luteinizing hormone stimulus
cellular response to cortisol stimulus
cellular response to progesterone stimulus
response to dexamethasone
response to indole-3-methanol
positive regulation of reactive oxygen species metabolic process
positive regulation of DNA biosynthetic process
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