Proteintech

CYP27A1 Polyclonal Antibody

CYP27A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CYP27A1 Antibody (14739-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14739-1-AP ( CYP27A1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

14739-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CYP27A1 Fusion Protein Ag6408 (183-531 aa encoded by BC051851)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DDFMTRLDQ LRAESASGNQ VSDMAQLFYY FALEAICYIL FEKRIGCLQR SIPEDTVTFV RSIGLMFQNS LYATFLPKWT RPVLPFWKRY LDGWNAIFSF GKKLIDEKLE DMEAQLQAAG PDGIQVSGYL HFLLASGQLS PREAMGSLPE LLMAGVDTTS NTLTWALYHL SKDPEIQEAL HEEVVGVVPA GQVPQHKDFA HMPLLKAVLK ETLRLYPVVP TNSRIIEKEI EVDGFLFPKN TQFVFCHYVV SRDPTAFSEP ESFQPHRWLR NSQPATPRIQ HPFGSVPFGY GVRACLGRRI AELEMQLLLA RLIQKYKVVL APETGELKSV ARIVLVPNKK VGLQFLQRQC (183-531 aa encoded by BC051851)

Target Information

CYP27A1 participates in the degradation of cholesterol to bile acids in both the classic and acidic pathways. [1] It is the initiating enzyme in the acidic pathway to bile acids, yielding oxysterols by introducing a hydroxyl group to the carbon at the 27 position in cholesterol. In the acidic pathway, it produces 27-hydroxycholesterol from cholesterol whereas in the classic or neutral pathway, it produces 3beta-hydroxy-5-cholestenoic acid. It is also involved in the metabolism of vitamin D3. While CYP27A1 is present in many different tissues, its function in these tissues is largely uncharacterized. In macrophages, 27-hydroxycholesterol generated by this enzyme may be helpful against the production of inflammatory factors associated with cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Supplemental Clostridium butyricum modulates lipid metabolism by reshaping the gut microbiota composition and bile acid profile in IUGR suckling piglets.

Journal of animal science and biotechnology

Zhang X,Yun Y,Lai Z,Ji S,Yu G,Xie Z,Zhang H,Zhong X,Wang T,Zhang L

Mon Mar 13 00:00:00 EDT 2023

Targeting mTOR/YY1 signaling pathway by quercetin through CYP7A1-mediated cholesterol-to-bile acids conversion alleviated type 2 diabetes mellitus induced hepatic lipid accumulation.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Yang T,Wang Y,Cao X,Peng Y,Huang J,Chen L,Pang J,Jiang Z,Qian S,Liu Y,Ying C,Wang T,Zhang F,Lu Q,Yin X

Mon May 01 00:00:00 EDT 2023

Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken.

Frontiers in veterinary science

Wu L,Liu X,Zhang A,Chen H,Zhao R,Jia Y

Sun Jun 04 00:00:00 EDT 2023

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fu Y,Feng H,Ding X,Meng QH,Zhang SR,Li J,Chao Y,Ji TT,Bi YH,Zhang WW,Chen Q,Zhang YH,Feng YL,Bian HM

Fri Jul 01 00:00:00 EDT 2022

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fu Y,Feng H,Ding X,Meng QH,Zhang SR,Li J,Chao Y,Ji TT,Bi YH,Zhang WW,Chen Q,Zhang YH,Feng YL,Bian HM

Fri Jul 01 00:00:00 EDT 2022

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fu Y,Feng H,Ding X,Meng QH,Zhang SR,Li J,Chao Y,Ji TT,Bi YH,Zhang WW,Chen Q,Zhang YH,Feng YL,Bian HM

Fri Jul 01 00:00:00 EDT 2022

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fu Y,Feng H,Ding X,Meng QH,Zhang SR,Li J,Chao Y,Ji TT,Bi YH,Zhang WW,Chen Q,Zhang YH,Feng YL,Bian HM

Fri Jul 01 00:00:00 EDT 2022

Oryzanol Attenuates High Fat and Cholesterol Diet-Induced Hyperlipidemia by Regulating the Gut Microbiome and Amino Acid Metabolism.

Journal of agricultural and food chemistry

Yan S,Chen J,Zhu L,Guo T,Qin D,Hu Z,Han S,Zhou Y,Akan OD,Wang J,Luo F,Lin Q

Wed Jun 01 00:00:00 EDT 2022

Bird's eye view analysis of in situ cholesterol metabolic pathways in breast cancer patients and its clinicopathological significance in their subtypes.

The Journal of steroid biochemistry and molecular biology

Kosaka S,Miyashita M,McNamala K,Nomura M,Shima H,Kawai M,Sato I,Harada-Shoji N,Ishida T,Choi MH,Sasano H

Fri Jul 01 00:00:00 EDT 2022

FXR Signaling-Mediated Bile Acid Metabolism Is Critical for Alleviation of Cholesterol Gallstones by Lactobacillus Strains.

Microbiology spectrum

Ye X,Huang D,Dong Z,Wang X,Ning M,Xia J,Shen S,Wu S,Shi Y,Wang J,Wan X

Wed Oct 26 00:00:00 EDT 2022

Dehydrodiconiferyl alcohol, a lignan from Herpetospermum pedunculosum, alleviates cholestasis by activating pathways associated with the farnesoid X receptor.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Wei X,Ma Y,Dong Z,Wang G,Lan X,Liao Z,Chen M

Fri Jan 01 00:00:00 EST 2021

Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Meng F,Zong W,Wei X,Tao Y,Wang G,Liao Z,Chen M

Thu Jul 01 00:00:00 EDT 2021

Chemical profile of Swertia mussotii Franch and its potential targets against liver fibrosis revealed by cross-platform metabolomics.

Journal of ethnopharmacology

Zhang Y,Zhou Q,Ding X,Ma J,Tan G

Mon Jun 28 00:00:00 EDT 2021

Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.

Cell research

Teng S,Li YE,Yang M,Qi R,Huang Y,Wang Q,Zhang Y,Chen S,Li S,Lin K,Cao Y,Ji Q,Gu Q,Cheng Y,Chang Z,Guo W,Wang P,Garcia-Bassets I,Lu ZJ,Wang D

Wed Jan 01 00:00:00 EST 2020

Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia.

Journal of lipid research

Mistry HD,Kurlak LO,Mansour YT,Zurkinden L,Mohaupt MG,Escher G

Thu Jun 01 00:00:00 EDT 2017

Maternal Betaine Supplementation throughout Gestation and Lactation Modifies Hepatic Cholesterol Metabolic Genes in Weaning Piglets via AMPK/LXR-Mediated Pathway and Histone Modification.

Nutrients

Cai D,Yuan M,Liu H,Pan S,Ma W,Hong J,Zhao R

Tue Oct 18 00:00:00 EDT 2016

Dysregulated bile acid synthesis, metabolism and excretion in a high fat-cholesterol diet-induced fibrotic steatohepatitis in rats.

Digestive diseases and sciences

Jia X,Naito H,Yetti H,Tamada H,Kitamori K,Hayashi Y,Wang D,Yanagiba Y,Wang J,Ikeda K,Yamori Y,Nakajima T

Thu Aug 01 00:00:00 EDT 2013

Dysregulated bile acid synthesis, metabolism and excretion in a high fat-cholesterol diet-induced fibrotic steatohepatitis in rats.

Digestive diseases and sciences

Jia X,Naito H,Yetti H,Tamada H,Kitamori K,Hayashi Y,Wang D,Yanagiba Y,Wang J,Ikeda K,Yamori Y,Nakajima T

Thu Aug 01 00:00:00 EDT 2013

Cholesterol-enriched diet causes age-related macular degeneration-like pathology in rabbit retina.

BMC ophthalmology

Dasari B,Prasanthi JR,Marwarha G,Singh BB,Ghribi O

Thu Aug 18 00:00:00 EDT 2011

Cholesterol-enriched diet causes age-related macular degeneration-like pathology in rabbit retina.

BMC ophthalmology

Dasari B,Prasanthi JR,Marwarha G,Singh BB,Ghribi O

Thu Aug 18 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; cholestanetriol 26-monooxygenase; cholesterol 27 hydroxylase; cyt; Cytochrome P-450C27/25; Cytochrome P450 27; cytochrome P450, 27; cytochrome P450, 27a1; cytochrome P450, family 27, subfamily A, polypeptide 1; cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1; Sterol 26-hydroxylase, mitochondrial; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase

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Gene Aliases: 1300013A03Rik; CP27; CTX; CYP27; CYP27A1; P450C27

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UniProt ID: (Human) Q02318, (Mouse) Q9DBG1, (Rat) P17178

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Entrez Gene ID: (Human) 1593, (Mouse) 104086, (Rat) 301517

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Function(s)

iron ion binding steroid hydroxylase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen heme binding monooxygenase activity oxidoreductase activity vitamin D3 25-hydroxylase activity cholesterol 26-hydroxylase activity metal ion binding cholestanetriol 26-monooxygenase activity oxygenase

Process(es)

bile acid biosynthetic process sterol metabolic process oxidation-reduction process cholesterol metabolic process calcitriol biosynthetic process from calciol steroid catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CYP27A1 Polyclonal Antibody

CYP27A1 Antibody (14739-1-AP) in IHC (P)

Product Details

14739-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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