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CYP2E1 Antibody (PA5-118842) in IHC (P)
Immunohistochemical analysis of CYP2E1 in Mouse liver tissue using a CYP2E1 polyclonal antibody (Product # PA5-118842). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Product Details
PA5-118842
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CYP2E1(Accession P05181), corresponding to amino acid residues Y381-S431.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2903342
Product Specific Information
Antibody detects endogenous levels of total Cytochrome P450 2E1.
Target Information
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 4-nitrophenol 2-hydroxylase; CP2E1; CYPIIE1; Cytochrome P450 2E1; cytochrome P450 subfamily 2e1 (ethanol-inducible); cytochrome P450, 2e1, ethanol inducible; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450, subfamily 2E, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; Cytochrome P450-ALC; Cytochrome P450-J; Cytochrome P450RLM6; flavoprotein-linked monooxygenase; microsomal monooxygenase; P450-J; P450RLM6; xenobiotic monooxygenase
Gene Aliases: CPE1; CYP2E; Cyp2e-1; CYP2E1; P450-J; P450C2E
UniProt ID: (Human) P05181, (Mouse) Q05421, (Rat) P05182
Entrez Gene ID: (Human) 1571, (Mouse) 13106, (Rat) 25086
monooxygenase activity
iron ion binding
arachidonic acid epoxygenase activity
steroid hydroxylase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
enzyme binding
heme binding
3-(3-hydroxyphenyl)propionate hydroxylase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
4-chlorobenzaldehyde oxidase activity
3,5-xylenol methylhydroxylase activity
phenylacetate hydroxylase activity
4-nitrophenol 4-monooxygenase activity
dimethyl sulfide monooxygenase activity
alpha-pinene monooxygenase [NADH] activity
phenanthrene 9,10-monooxygenase activity
1-hydroxy-2-naphthoate hydroxylase activity
toluene 4-monooxygenase activity
xylene monooxygenase activity
dibenzothiophene monooxygenase activity
6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity
chlorophenol 4-monooxygenase activity
carbon disulfide oxygenase activity
toluene 2-monooxygenase activity
1-hydroxy-2-oxolimonene 1,2-monooxygenase activity
phenanthrene 1,2-monooxygenase activity
tetrahydrofuran hydroxylase activity
styrene monooxygenase activity
toluene-4-sulfonate monooxygenase activity
toluene-sulfonate methyl-monooxygenase activity
3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity
2-hydroxy-phenylacetate hydroxylase activity
2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase activity
phenanthrene 3,4-monooxygenase activity
toluene 3-monooxygenase activity
4-hydroxyphenylacetate,NADH:oxygen oxidoreductase (3-hydroxylating) activity
limonene monooxygenase activity
2-methylnaphthalene hydroxylase activity
1-methylnaphthalene hydroxylase activity
bisphenol A hydroxylase A activity
salicylate 5-hydroxylase activity
isobutylamine N-hydroxylase activity
branched-chain dodecylbenzene sulfonate monooxygenase activity
3-HSA hydroxylase activity
4-hydroxypyridine-3-hydroxylase activity
2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase activity
6-hydroxynicotinate 3-monooxygenase activity
metal ion binding
tocotrienol omega-hydroxylase activity
thalianol hydroxylase activity
FMN binding
oxidoreductase
oxygenase
metabolite interconversion enzyme
triglyceride metabolic process
xenobiotic metabolic process
steroid metabolic process
response to ozone
response to organonitrogen compound
monoterpenoid metabolic process
drug metabolic process
carbon tetrachloride metabolic process
benzene metabolic process
epoxygenase P450 pathway
halogenated hydrocarbon metabolic process
response to drug
response to ethanol
heterocycle metabolic process
oxidation-reduction process
exogenous drug catabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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