Proteintech

CYP46A1 Polyclonal Antibody

CYP46A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CYP46A1 Antibody (12486-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using (Product # 12486-1-AP) (CYP46A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CYP46A1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12486-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Rat, Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CYP46A1 Fusion Protein Ag3116 (201-500 aa encoded by BC022539)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TSMLLGAQK PLSQAVKLML EGITASRNTL AKFLPGKRKQ LREVRESIRF LRQVGRDWVQ RRREALKRGE EVPADILTQI LKAEEGAQDD EGLLDNFVTF FIAGHETSAN HLAFTVMELS RQPEIVARLQ AEVDEVIGSK RYLDFEDLGR LQYLSQVLKE SLRLYPPAWG TFRLLEEETL IDGVRVPGNT PLLFSTYVMG RMDTYFEDPL TFNPDRFGPG APKPRFTYFP FSLGHRSCIG QQFAQMEVKV VMAKLLQRLE FRLVPGQRFG LQEQATLKPL DPVLCTLRPR GWQPAPPPPP C (201-500 aa encoded by BC022539)

Target Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cholesterol-24-hydroxylase (CYP46) in the old brain: Analysis of positive populations and factors triggering its expression in astrocytes.

The Journal of comparative neurology

Cataldi GG,Elorza SD,Toledano-Zaragoza A,de Olmos S,Cragnolini AB,Martín MG

Wed Feb 01 00:00:00 EST 2023

The PPARα/CYP4A14 bile acid pathway is associated with lipid metabolism disorders caused by low birth weight with high-fat diet.

Food & nutrition research

Zhou F,Yang L,Sun W,Wang X,Guo N,Ma H,Yang L

Fri Feb 17 00:00:00 EST 2023

ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.

Nature communications

Zhao Y,Hu D,Wang R,Sun X,Ropelewski P,Hubler Z,Lundberg K,Wang Q,Adams DJ,Xu R,Qi X

Wed Mar 02 00:00:00 EST 2022

Bird's eye view analysis of in situ cholesterol metabolic pathways in breast cancer patients and its clinicopathological significance in their subtypes.

The Journal of steroid biochemistry and molecular biology

Kosaka S,Miyashita M,McNamala K,Nomura M,Shima H,Kawai M,Sato I,Harada-Shoji N,Ishida T,Choi MH,Sasano H

Fri Jul 01 00:00:00 EDT 2022

Transcription of cytochrome P450 46A1 in NIH3T3 cells is negatively regulated by FBS.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Shinohara Y,Ando H,Maekawa M,Arai M,Horibata Y,Satou M,Jojima T,Usui I,Aso Y,Sugimoto H

Wed Jun 01 00:00:00 EDT 2022

Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma.

Oncogene

Yang R,Zhao Y,Gu Y,Yang Y,Gao X,Yuan Y,Xiao L,Zhang J,Sun C,Yang H,Qin J,Li J,Zhang F,Zhang L,Ye J

Thu Oct 01 00:00:00 EDT 2020

NPC1 enables cholesterol mobilization during long-term potentiation that can be restored in Niemann-Pick disease type C by CYP46A1 activation.

EMBO reports

Mitroi DN,Pereyra-Gómez G,Soto-Huelin B,Senovilla F,Kobayashi T,Esteban JA,Ledesma MD

Tue Nov 05 00:00:00 EST 2019

24(S)-Hydroxycholesterol protects the ex vivo rat retina from injury by elevated hydrostatic pressure.

Scientific reports

Ishikawa M,Yoshitomi T,Zorumski CF,Izumi Y

Thu Sep 22 00:00:00 EDT 2016

24(S)-Hydroxycholesterol protects the ex vivo rat retina from injury by elevated hydrostatic pressure.

Scientific reports

Ishikawa M,Yoshitomi T,Zorumski CF,Izumi Y

Thu Sep 22 00:00:00 EDT 2016

H63D mutation in hemochromatosis alters cholesterol metabolism and induces memory impairment.

Neurobiology of aging

Ali-Rahmani F,Grigson PS,Lee S,Neely E,Connor JR,Schengrund CL

Sun Jun 01 00:00:00 EDT 2014

H63D mutation in hemochromatosis alters cholesterol metabolism and induces memory impairment.

Neurobiology of aging

Ali-Rahmani F,Grigson PS,Lee S,Neely E,Connor JR,Schengrund CL

Sun Jun 01 00:00:00 EDT 2014

Differentiation of mouse embryonic stem cells into hepatocytes induced by a combination of cytokines and sodium butyrate.

Journal of cellular biochemistry

Zhou M,Li P,Tan L,Qu S,Ying QL,Song H

Mon Feb 15 00:00:00 EST 2010

Differentiation of mouse embryonic stem cells into hepatocytes induced by a combination of cytokines and sodium butyrate.

Journal of cellular biochemistry

Zhou M,Li P,Tan L,Qu S,Ying QL,Song H

Mon Feb 15 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CH24H; Cholesterol 24-hydroxylase; Cholesterol 24-monooxygenase; Cholesterol 24S-hydroxylase; cholestrol 24-hydroxylase; Cytochrome P450 46A1; cytochrome P450, 46 (cholesterol 24-hydroxylase); cytochrome P450, 46a1 (cholesterol 24-hydroxylase); cytochrome P450, family 46, subfamily A, polypeptide 1; cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)

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Gene Aliases: CP46; CYP46; CYP46A1

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UniProt ID: (Human) Q9Y6A2, (Mouse) Q9WVK8

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Entrez Gene ID: (Human) 10858, (Rat) 362782, (Mouse) 13116

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Function(s)

iron ion binding steroid hydroxylase activity heme binding cholesterol 24-hydroxylase activity monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding oxidoreductase oxygenase metabolite interconversion enzyme

Process(es)

bile acid biosynthetic process cholesterol catabolic process xenobiotic metabolic process nervous system development sterol metabolic process oxidation-reduction process lipid metabolic process steroid metabolic process cholesterol metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CYP46A1 Polyclonal Antibody

CYP46A1 Antibody (12486-1-AP) in IHC (P)

Product Details

12486-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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