Proteintech

CYP51A1 Polyclonal Antibody

CYP51A1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

CYP51A1 Antibody (13431-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-3 cells using 13431-1-AP (CYP51A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CYP51A1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13431-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 20 publications 20 publications

Immunocytochemistry (ICC/IF)

1:50

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CYP51A1 Fusion Protein Ag4235 (146-509 aa encoded by BC032322)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GKGVAYDVP NPVFLEQKKM LKSGLNIAHF KQHVSIIEKE TKEYFESWGE SGEKNVFEAL SELIILTASH CLHGKEIRSQ LNEKVAQLYA DLDGGFSHAA WLLPGWLPLP SFRRRDRAHR EIKDIFYKAI QKRRQSQEKI DDILQTLLDA TYKDGRPLTD DEVAGMLIGL LLAGQHTSST TSAWMGFFLA RDKTLQKKCY LEQKTVCGEN LPPLTYDQLK DLNLLDRCIK ETLRLRPPVM IMMRMARTPQ TVAGYTIPPG HQVCVSPTVN QRLKDSWVER LDFNPDRYLQ DNPASGEKFA YVPFGAGRHR CIGENFAYVQ IKTIWSTMLR LYEFDLIDGY FPTVNYTTMI HTPENPVIRY KRRSK (146-509 aa encoded by BC032322)

Target Information

P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYP51A1 (cytochrome P450, family 51, subfamily A, polypeptide 1), also known as LDM (lanosterol 14-alpha demethylase) or cytochrome P450-14DM, is a 503 amino acid protein localized to endoplasmic reticulum membrane. CYP51A1 is an important enzyme for zymosterol and steroid biosynthesis. CYP51A1 catalyzes C14-demethylation of lanosterol, transforming lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. CYP51A1 is ubiquitously expressed, with highest levels found in liver, ovary, testis, lung, kidney and prostate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bicyclol attenuates high fat diet-induced non-alcoholic fatty liver disease/non-alcoholic steatohepatitis through modulating multiple pathways in mice.

Frontiers in pharmacology

Wu J,Jia S,Xu B,Yao X,Shao J,Yao J,Cen D,Yao X

Mon Jun 05 00:00:00 EDT 2023

Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions.

Nature communications

Fan S,Yan Y,Xia Y,Zhou Z,Luo L,Zhu M,Han Y,Yao D,Zhang L,Fang M,Peng L,Yu J,Liu Y,Gao X,Guan H,Li H,Wang C,Wu X,Zhu H,Cao Y,Huang C

Thu Jun 08 00:00:00 EDT 2023

Effects and potential mechanism of Ca²⁺/calmodulin‑dependent protein kinase II pathway inhibitor KN93 on the development of ovarian follicle.

International journal of molecular medicine

Yu J,Xie X,Ma Y,Yang Y,Wang C,Xia G,Ding X,Liu X

Sat Oct 01 00:00:00 EDT 2022

Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro.

Stem cells international

Li JQ,Jiang HJ,Su XY,Feng L,Zhan NZ,Li SS,Chen ZJ,Chang BH,Cheng PZ,Yang L,Pei GX

Tue Jan 25 00:00:00 EST 2022

POST1/C12ORF49 regulates the SREBP pathway by promoting site-1 protease maturation.

Protein & cell

Xiao J,Xiong Y,Yang LT,Wang JQ,Zhou ZM,Dong LW,Shi XJ,Zhao X,Luo J,Song BL

Thu Apr 01 00:00:00 EDT 2021

Vascular biotransformation of organic nitrates is independent of cytochrome P450 monooxygenases.

British journal of pharmacology

Lopez M,Malacarne PF,Gajos-Draus A,Ding X,Daiber A,Lundberg JO,Offermanns S,Brandes RP,Rezende F

Thu Apr 01 00:00:00 EDT 2021

MMAB promotes negative feedback control of cholesterol homeostasis.

Nature communications

Goedeke L,Canfrán-Duque A,Rotllan N,Chaube B,Thompson BM,Lee RG,Cline GW,McDonald JG,Shulman GI,Lasunción MA,Suárez Y,Fernández-Hernando C

Mon Nov 08 00:00:00 EST 2021

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Neuron

Li X,Zhang J,Li D,He C,He K,Xue T,Wan L,Zhang C,Liu Q

Wed Mar 17 00:00:00 EDT 2021

The cholesterol synthesis enzyme lanosterol 14α-demethylase is post-translationally regulated by the E3 ubiquitin ligase MARCH6.

The Biochemical journal

Scott NA,Sharpe LJ,Capell-Hattam IM,Gullo SJ,Luu W,Brown AJ

Fri Jan 31 00:00:00 EST 2020

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Molecular cell

van de Weijer ML,Krshnan L,Liberatori S,Guerrero EN,Robson-Tull J,Hahn L,Lebbink RJ,Wiertz EJHJ,Fischer R,Ebner D,Carvalho P

Thu Oct 15 00:00:00 EDT 2020

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Molecular cell

van de Weijer ML,Krshnan L,Liberatori S,Guerrero EN,Robson-Tull J,Hahn L,Lebbink RJ,Wiertz EJHJ,Fischer R,Ebner D,Carvalho P

Thu Sep 03 00:00:00 EDT 2020

Elevated cellular cholesterol in Familial Alzheimer's presenilin 1 mutation is associated with lipid raft localization of β-amyloid precursor protein.

PloS one

Cho YY,Kwon OH,Park MK,Kim TW,Chung S

Mon Nov 18 00:00:00 EST 2019

Endogenous sterol intermediates of the mevalonate pathway regulate HMGCR degradation and SREBP-2 processing.

Journal of lipid research

Chen L,Ma MY,Sun M,Jiang LY,Zhao XT,Fang XX,Man Lam S,Shui GH,Luo J,Shi XJ,Song BL

Tue Oct 01 00:00:00 EDT 2019

Pharmacogenomics of Chemically Distinct Classes of Keap1-Nrf2 Activators Identify Common and Unique Gene, Protein, and Pathway Responses In Vivo.

Molecular pharmacology

Wible RS,Tran QT,Fathima S,Sutter CH,Kensler TW,Sutter TR

Sun Apr 01 00:00:00 EDT 2018

Pharmacogenomics of Chemically Distinct Classes of Keap1-Nrf2 Activators Identify Common and Unique Gene, Protein, and Pathway Responses In Vivo.

Molecular pharmacology

Wible RS,Tran QT,Fathima S,Sutter CH,Kensler TW,Sutter TR

Sun Apr 01 00:00:00 EDT 2018

Nitric oxide stimulates cellular degradation of human CYP51A1, the highly conserved lanosterol 14α-demethylase.

The Biochemical journal

Park JW,Byrd A,Lee CM,Morgan ET

Thu Sep 14 00:00:00 EDT 2017

Nitric oxide stimulates cellular degradation of human CYP51A1, the highly conserved lanosterol 14α-demethylase.

The Biochemical journal

Park JW,Byrd A,Lee CM,Morgan ET

Thu Sep 14 00:00:00 EDT 2017

Lanosterol Modulates TLR4-Mediated Innate Immune Responses in Macrophages.

Cell reports

Araldi E,Fernández-Fuertes M,Canfrán-Duque A,Tang W,Cline GW,Madrigal-Matute J,Pober JS,Lasunción MA,Wu D,Fernández-Hernando C,Suárez Y

Tue Jun 27 00:00:00 EDT 2017

Lanosterol Modulates TLR4-Mediated Innate Immune Responses in Macrophages.

Cell reports

Araldi E,Fernández-Fuertes M,Canfrán-Duque A,Tang W,Cline GW,Madrigal-Matute J,Pober JS,Lasunción MA,Wu D,Fernández-Hernando C,Suárez Y

Tue Jun 27 00:00:00 EDT 2017

Antifungal drug itraconazole targets VDAC1 to modulate the AMPK/mTOR signaling axis in endothelial cells.

Proceedings of the National Academy of Sciences of the United States of America

Head SA,Shi W,Zhao L,Gorshkov K,Pasunooti K,Chen Y,Deng Z,Li RJ,Shim JS,Tan W,Hartung T,Zhang J,Zhao Y,Colombini M,Liu JO

Tue Dec 29 00:00:00 EST 2015

Protective effects of dioscin against alcohol-induced liver injury.

Archives of toxicology

Xu T,Zheng L,Xu L,Yin L,Qi Y,Xu Y,Han X,Peng J

Sat Mar 01 00:00:00 EST 2014

Protective effects of dioscin against alcohol-induced liver injury.

Archives of toxicology

Xu T,Zheng L,Xu L,Yin L,Qi Y,Xu Y,Han X,Peng J

Sat Mar 01 00:00:00 EST 2014

Protective effects of dioscin against alcohol-induced liver injury.

Archives of toxicology

Xu T,Zheng L,Xu L,Yin L,Qi Y,Xu Y,Han X,Peng J

Sat Mar 01 00:00:00 EST 2014

CYB5D2 requires heme-binding to regulate HeLa cell growth and confer survival from chemotherapeutic agents.

PloS one

Bruce A,Rybak AP

Tue Nov 04 00:00:00 EST 2014

CYB5D2 requires heme-binding to regulate HeLa cell growth and confer survival from chemotherapeutic agents.

PloS one

Bruce A,Rybak AP

Tue Nov 04 00:00:00 EST 2014

Suppression of cytochrome P450 reductase (POR) expression in hepatoma cells replicates the hepatic lipidosis observed in hepatic POR-null mice.

Drug metabolism and disposition: the biological fate of chemicals

Porter TD,Banerjee S,Stolarczyk EI,Zou L

Wed Jun 01 00:00:00 EDT 2011

Suppression of cytochrome P450 reductase (POR) expression in hepatoma cells replicates the hepatic lipidosis observed in hepatic POR-null mice.

Drug metabolism and disposition: the biological fate of chemicals

Porter TD,Banerjee S,Stolarczyk EI,Zou L

Wed Jun 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CYP51; CYP51A1; CYPLI; Cytochrom P450 Lanosterol 14 alpha-demethylase; Cytochrome P450 51A1; cytochrome P450 Lanosterol 14 alpha-demethylase; cytochrome P450, 51; cytochrome P450, 51 (lanosterol 14-alpha-demethylase); cytochrome P450, family 51, subfamily A, polypeptide 1; cytochrome P450, subfamily 51; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Lanosterol 14-alpha demethylase; lanosterol 14-alpha-demethylase; LDM; P450 14DM; P450-14DM; P45014DM; P450LI; Sterol 14-alpha demethylase; Sterol 14-alpha demethylase P450

View more View less

Gene Aliases: AI426508; CP51; CYP51; CYP51A1; CYPL1; CYPLI; LDM; P450-14DM; P450L1; P450LI; RATCP14DM

View more View less

UniProt ID: (Human) Q16850, (Mouse) Q8K0C4, (Rat) Q64549

View more View less

Entrez Gene ID: (Human) 1595, (Mouse) 13121, (Rat) 25427

View more View less

Function(s)

iron ion binding sterol 14-demethylase activity heme binding monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding oxygenase

Process(es)

steroid biosynthetic process cholesterol biosynthetic process sterol metabolic process cholesterol biosynthetic process via 24,25-dihydrolanosterol oxidation-reduction process demethylation lipid metabolic process steroid metabolic process cholesterol metabolic process sterol biosynthetic process regulation of cholesterol biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CYP51A1 Polyclonal Antibody

CYP51A1 Antibody (13431-1-AP) in ICC/IF

Product Details

13431-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!