Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
CYP51A1 Antibody (PA5-76873) in IHC (P)
Immunohistochemistry analysis of CYP51A1 in paraffin-embedded human thyroid cancer. Samples were incubated with CYP51A1 polyclonal antibody (Product # PA5-76873) using a dilution of 1:50. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-76873
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human CYP51A1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2720600
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYP51A1 (cytochrome P450, family 51, subfamily A, polypeptide 1), also known as LDM (lanosterol 14-alpha demethylase) or cytochrome P450-14DM, is a 503 amino acid protein localized to endoplasmic reticulum membrane. CYP51A1 is an important enzyme for zymosterol and steroid biosynthesis. CYP51A1 catalyzes C14-demethylation of lanosterol, transforming lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. CYP51A1 is ubiquitously expressed, with highest levels found in liver, ovary, testis, lung, kidney and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CYP51; CYP51A1; CYPLI; Cytochrom P450 Lanosterol 14 alpha-demethylase; Cytochrome P450 51A1; cytochrome P450 Lanosterol 14 alpha-demethylase; cytochrome P450, 51; cytochrome P450, 51 (lanosterol 14-alpha-demethylase); cytochrome P450, family 51, subfamily A, polypeptide 1; cytochrome P450, subfamily 51; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Lanosterol 14-alpha demethylase; lanosterol 14-alpha-demethylase; LDM; P450 14DM; P450-14DM; P45014DM; P450LI; Sterol 14-alpha demethylase; Sterol 14-alpha demethylase P450
Gene Aliases: AI426508; CP51; CYP51; CYP51A1; CYPL1; CYPLI; LDM; P450-14DM; P450L1; P450LI; RATCP14DM
UniProt ID: (Human) Q16850, (Mouse) Q8K0C4, (Rat) Q64549
Entrez Gene ID: (Human) 1595, (Mouse) 13121, (Rat) 25427
steroid biosynthetic process
cholesterol biosynthetic process
sterol metabolic process
cholesterol biosynthetic process via 24,25-dihydrolanosterol
oxidation-reduction process
demethylation
lipid metabolic process
steroid metabolic process
cholesterol metabolic process
sterol biosynthetic process
regulation of cholesterol biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support