Proteintech

CYP7B1 Polyclonal Antibody

CYP7B1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

CYP7B1 Antibody (24889-1-AP) in ICC/IF

Immunofluorescent analysis of HEK-293 cells using 24889-1-AP (CYP7B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CYP7B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

24889-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CYP7B1 Fusion Protein Ag19374 (96-403 aa encoded by BC136574)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: PFQYQLVIK NHKQLSFRVF SNKLLEKAFS ISQLQKNHDM NDELHLCYQF LQGKSLDILL ESMMQNLKQV FEPQLLKTTS WDTAELYPFC SSIIFEITFT TIYGKVIVCD NNKFISELRD DFLKFDDKFA YLVSNIPIEL LGNVKSIREK IIKCFSSEKL AKMQGWSEVF QSRQDVLEKY YVHEDLEIGA HHLGFLWASV ANTIPTMFWA MYYLLRHPEA MAAVRDEIDR LLQSTGQKKG SGFPIHLTRE QLDSLICLES SIFEALRLSS YSTTIRFVEE DLTLSSETGD YCVRKGDLVA IFPPVLHGD (96-403 aa encoded by BC136574)

Target Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen.

Nature microbiology

Xie J,Li H,Zhang X,Yang T,Yue M,Zhang Y,Chen S,Cui N,Yuan C,Li J,Zhu SJ,Liu W

Sun Jan 01 00:00:00 EST 2023

Stigmasterol Protects Against Steatohepatitis Induced by High-Fat and High-Cholesterol Diet in Mice by Enhancing the Alternative Bile Acid Synthesis Pathway.

The Journal of nutrition

Xin Y,Li X,Zhu X,Lin X,Luo M,Xiao Y,Ruan Y,Guo H

Sat Jul 01 00:00:00 EDT 2023

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fu Y,Feng H,Ding X,Meng QH,Zhang SR,Li J,Chao Y,Ji TT,Bi YH,Zhang WW,Chen Q,Zhang YH,Feng YL,Bian HM

Fri Jul 01 00:00:00 EDT 2022

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fu Y,Feng H,Ding X,Meng QH,Zhang SR,Li J,Chao Y,Ji TT,Bi YH,Zhang WW,Chen Q,Zhang YH,Feng YL,Bian HM

Fri Jul 01 00:00:00 EDT 2022

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fu Y,Feng H,Ding X,Meng QH,Zhang SR,Li J,Chao Y,Ji TT,Bi YH,Zhang WW,Chen Q,Zhang YH,Feng YL,Bian HM

Fri Jul 01 00:00:00 EDT 2022

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fu Y,Feng H,Ding X,Meng QH,Zhang SR,Li J,Chao Y,Ji TT,Bi YH,Zhang WW,Chen Q,Zhang YH,Feng YL,Bian HM

Fri Jul 01 00:00:00 EDT 2022

Gypenosides Prevent and Dissolve Cholesterol Gallstones by Modulating the Homeostasis of Cholesterol and Bile Acids.

Frontiers in medicine

Zhuang Q,Cheng J,Xia J,Ning M,Wu S,Shen S,Shi Y,Huang D,Dong Z,Wan X

Sat Apr 23 00:00:00 EDT 2022

FXR Signaling-Mediated Bile Acid Metabolism Is Critical for Alleviation of Cholesterol Gallstones by Lactobacillus Strains.

Microbiology spectrum

Ye X,Huang D,Dong Z,Wang X,Ning M,Xia J,Shen S,Wu S,Shi Y,Wang J,Wan X

Wed Oct 26 00:00:00 EDT 2022

Dysregulated oxalate metabolism is a driver and therapeutic target in atherosclerosis.

Cell reports

Liu Y,Zhao Y,Shukha Y,Lu H,Wang L,Liu Z,Liu C,Zhao Y,Wang H,Zhao G,Liang W,Fan Y,Chang L,Yurdagul A,Pattillo CB,Orr AW,Aviram M,Wen B,Garcia-Barrio MT,Zhang J,Liu W,Sun D,Hayek T,Chen YE,Rom O

Tue Jul 27 00:00:00 EDT 2021

Gut microbiota mediates the effects of curcumin on enhancing Ucp1-dependent thermogenesis and improving high-fat diet-induced obesity.

Food & function

Han Z,Yao L,Zhong Y,Xiao Y,Gao J,Zheng Z,Fan S,Zhang Z,Gong S,Chang S,Cui X,Cai J

Wed Jul 21 00:00:00 EDT 2021

Deficiency of coiled-coil domain containing 80 increases plasma cholesterol by decreasing fecal sterols excretion in hypercholesterolemic mice.

The Journal of nutritional biochemistry

Li W,Sun Z,Wu M,Deng Z,Zheng M,Kuang Z,Liu Y,He G

Wed Dec 01 00:00:00 EST 2021

Astragalus Polysaccharides Ameliorate Diet-Induced Gallstone Formation by Modulating Synthesis of Bile Acids and the Gut Microbiota.

Frontiers in pharmacology

Zhuang Q,Ye X,Shen S,Cheng J,Shi Y,Wu S,Xia J,Ning M,Dong Z,Wan X

Tue Jul 20 00:00:00 EDT 2021

Downregulation of GPR183 on infection restricts the early infection and intracellular replication of mycobacterium tuberculosis in macrophage.

Microbial pathogenesis

Tang J,Shi Y,Zhan L,Qin C

Sat Aug 01 00:00:00 EDT 2020

Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition.

Journal of lipid research

Kakiyama G,Marques D,Martin R,Takei H,Rodriguez-Agudo D,LaSalle SA,Hashiguchi T,Liu X,Green R,Erickson S,Gil G,Fuchs M,Suzuki M,Murai T,Nittono H,Hylemon PB,Zhou H,Pandak WM

Tue Dec 01 00:00:00 EST 2020

Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.

Biochemical pharmacology

Chen Y,Zhou J,Xu S,Liu M,Wang M,Ma Y,Zhao M,Wang Z,Guo Y,Zhao L

Sun Dec 01 00:00:00 EST 2019

Hepatic, Metabolic, and Toxicity Evaluation of Repeated Oral Administration of SnS2 Nanoflowers in Mice.

Toxicological sciences : an official journal of the Society of Toxicology

Bai D,Li Q,Xiong Y,Wang C,Shen P,Bai L,Yuan L,Wu P

Wed Aug 01 00:00:00 EDT 2018

Hepatic, Metabolic, and Toxicity Evaluation of Repeated Oral Administration of SnS2 Nanoflowers in Mice.

Toxicological sciences : an official journal of the Society of Toxicology

Bai D,Li Q,Xiong Y,Wang C,Shen P,Bai L,Yuan L,Wu P

Wed Aug 01 00:00:00 EDT 2018

Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zhong D,Xie Z,Huang B,Zhu S,Wang G,Zhou H,Lin S,Lin Z,Yang B

Fri Oct 12 00:00:00 EDT 2018

Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zhong D,Xie Z,Huang B,Zhu S,Wang G,Zhou H,Lin S,Lin Z,Yang B

Fri Oct 12 00:00:00 EDT 2018

Hydroxylation and sulfation of sex steroid hormones in inflammatory liver.

Journal of biomedical research

Lee SR,Lee SY,Kim SY,Ryu SY,Park BK,Hong EJ

Tue Sep 26 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: 24-hydroxycholesterol 7-alpha-hydroxylase; 25-hydroxycholesterol 7-alpha-hydroxylase; 25/26-hydroxycholesterol 7-alpha-hydroxylase; 3-hydroxysteroid 7-alpha hydroxylase; Cytochrome P450 7B1; cytochrome P450, 7b1; cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1; HCT-1; Hippocampal transcript 1 protein; Oxysterol 7-alpha-hydroxylase

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Gene Aliases: AW261589; CBAS3; CP7B; CYP7B1; D3Ertd552e; hct-1; SPG5A

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UniProt ID: (Human) O75881, (Mouse) Q60991

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Entrez Gene ID: (Human) 9420, (Mouse) 13123

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Function(s)

iron ion binding oxysterol 7-alpha-hydroxylase activity heme binding 25-hydroxycholesterol 7alpha-hydroxylase activity monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding oxygenase

Process(es)

bile acid biosynthetic process cholesterol metabolic process sterol metabolic process negative regulation of intracellular estrogen receptor signaling pathway B cell chemotaxis positive regulation of epithelial cell proliferation oxidation-reduction process prostate gland epithelium morphogenesis lipid metabolic process digestion memory circadian rhythm steroid metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CYP7B1 Polyclonal Antibody

CYP7B1 Antibody (24889-1-AP) in ICC/IF

Product Details

24889-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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