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Invitrogen
CaMKII alpha Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
CaMKII alpha Antibody (PA5-17095) in WB
Western blot was performed using Anti-CaMKII alpha Polyclonal Antibody, (Product # PA5-17095) and band at ~50 kDa corresponding to CaMKII alpha was observed in brain tissue but not in heart tissue as reported. Tissue extracts (30 µg lysate) of Moose Brain (Lane 1), Rat Brain (Lane 2), Mouse Heart (Lane 3) and Rat Heart (Lane 4) were electrophoresed using Novex® NuPAGE® 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (P... View More
Product Details
PA5-17095
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the human sequence of CaMKII-a
Conjugate
Form
Liquid
Concentration
30 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11002191
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+/calmodulin (CaM) binding and autophosphorylation and has been implicated in regulation of the cell cycle and transcription. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha CaM kinase II; alpha-CaMKII; Ca2+/calmodulin-dependent protein kinase II alpha; Calci; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaM-kinase II alpha chain; CaMK II; CaMK-II alpha subunit; caMK-II subunit alpha; CaMKIINalpha; EC 2.7.11.17; OTTHUMP00000165787; OTTHUMP00000165788; Phospho-CaMKI
Gene Aliases: CAMK2A; CAMKA; CaMKII; KIAA0968; mKIAA0968; PK2CDD; PKCCD; R74975
UniProt ID: (Human) Q9UQM7, (Mouse) P11798, (Rat) P11275
Entrez Gene ID: (Human) 815, (Mouse) 12322, (Rat) 25400
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
Ras guanyl-nucleotide exchange factor activity
protein binding
calmodulin binding
ATP binding
kinase activity
glutamate receptor binding
protein homodimerization activity
nucleotide binding
protein kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
GTPase activating protein binding
non-receptor serine/threonine protein kinase
G1/S transition of mitotic cell cycle
MAPK cascade
response to ischemia
protein phosphorylation
calcium ion transport
Wnt signaling pathway, calcium modulating pathway
positive regulation of cardiac muscle cell apoptotic process
peptidyl-serine phosphorylation
angiotensin-activated signaling pathway
positive regulation of GTPase activity
protein autophosphorylation
regulation of neurotransmitter secretion
regulation of neuronal synaptic plasticity
positive regulation of NF-kappaB transcription factor activity
positive regulation of calcium ion transport
interferon-gamma-mediated signaling pathway
regulation of cellular response to heat
regulation of mitochondrial membrane permeability involved in apoptotic process
phosphorylation
ionotropic glutamate receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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