Invitrogen

Calnexin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

15 References

View all (50) Calnexin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Calnexin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 24

Calnexin Antibody (PA5-34754) in ICC/IF

Immunofluorescence analysis of Calnexin was performed using 70% confluent log phase HeLa cells treated with Thapsigargin (1uM for 24hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Calnexin Polyclonal Antibody (Product # PA5-34754) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Ale... View More

Calnexin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Calnexin Antibody in Immunoprecipitation (IP)

Calnexin Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-34754

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 8 publications 8 publications

Immunoprecipitation (IP)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Sheep, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 525 and 592 of Calnexin (Uniprot ID#P27824)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.46 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2552106

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, A375, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2, KN73.

Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Pig (100%), Bovine (100%).

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intranasally administered extracellular vesicles from human induced pluripotent stem cell-derived neural stem cells quickly incorporate into neurons and microglia in 5xFAD mice.

Frontiers in aging neuroscience

Attaluri S,Jaimes Gonzalez J,Kirmani M,Vogel AD,Upadhya R,Kodali M,Madhu LN,Rao S,Shuai B,Babu RS,Huard C,Shetty AK

Published figure using Calnexin polyclonal antibody (Product # PA5-34754) in Western Blot

Thu Jul 27 00:00:00 EDT 2023

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

PA5-34754 was used in Western Blotting to find that ADSCs-EVs transferred miR-22-3p to pneumonocytes and reduced apoptosis, inflammation, and oxidative stress through KDM6B/HMGA2, mitigating OSAHS-LI progression.

Wed Mar 01 00:00:00 EST 2023

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

Wed Mar 01 00:00:00 EST 2023

Quantitative differentiation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability.

Disease models & mechanisms

Scelsi HF,Hill KR,Barlow BM,Martin MD,Lieberman RL

PA5-34754 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to motivate genotyping MYOC in patients for clinical monitoring of this widespread, painless and irreversible ocular disease.

Sun Jan 01 00:00:00 EST 2023

Cardiomyopathy related desmocollin-2 prodomain variants affect the intracellular cadherin transport and processing.

Frontiers in cardiovascular medicine

Pohl GM,Göz M,Gaertner A,Brodehl A,Cimen T,Saguner AM,Schulze-Bahr E,Walhorn V,Anselmetti D,Milting H

PA5-34754 was used in Immunocytochemistry to reveal that all analyzed positions in the prodomain are critical for the intracellular transport.

Tue Jun 06 00:00:00 EDT 2023

Human Papillomavirus Minor Capsid Protein L2 Mediates Intracellular Trafficking into and Passage beyond the Endoplasmic Reticulum.

Microbiology spectrum

Morante AV,Baboolal DD,Simon X,Pan EC,Meneses PI

PA5-34754 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to illustrate that through modification of the 43-DQILQ-47 sequence in pseudovirions, the importance of the 43-DQILQ-47 sequence in the trafficking of the virus beyond the endoplasmic reticulum.

Wed Jun 29 00:00:00 EDT 2022

Human Papillomavirus Minor Capsid Protein L2 Mediates Intracellular Trafficking into and Passage beyond the Endoplasmic Reticulum.

Microbiology spectrum

Morante AV,Baboolal DD,Simon X,Pan EC,Meneses PI

Wed Jun 29 00:00:00 EDT 2022

Implantable Electroceutical Approach Improves Myelination by Restoring Membrane Integrity in a Mouse Model of Peripheral Demyelinating Neuropathy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Intisar A,Shin HY,Kim WH,Kang HG,Kim MY,Kim YS,Cho Y,Mo YJ,Lim H,Lee S,Lu QR,Lee YI,Kim MS

PA5-34754 was used in Immunocytochemistry-immunofluorescence to demonstrate, for the first time, that an electroceutical approach with the optimal ES condition has the potential to treat CMT disease and restore impaired myelin membrane integrity, shifting the paradigm toward practical interventions for peripheral demyelinating neuropathies.

Tue Nov 01 00:00:00 EDT 2022

The Autism Spectrum Disorder-Associated Bacterial Metabolite p-Cresol Derails the Neuroimmune Response of Microglial Cells Partially via Reduction of ADAM17 and ADAM10.

International journal of molecular sciences

Zheng Y,Prince NZ,Peralta Marzal LN,Ahmed S,Garssen J,Perez Pardo P,Kraneveld AD

PA5-34754 was used in Western Blotting to demonstrate a partial role of ADAM10 and ADAM17 in the derailed innate immune response of microglial cells associated with pCS-induced ASD pathogenesis.

Tue Sep 20 00:00:00 EDT 2022

The Autophagy Receptor TAX1BP1 (T6BP) improves antigen presentation by MHC-II molecules.

EMBO reports

Sarango G,Richetta C,Pereira M,Kumari A,Ghosh M,Bertrand L,Pionneau C,Le Gall M,Grégoire S,Jeger-Madiot R,Rosoy E,Subra F,Delelis O,Faure M,Esclatine A,Graff-Dubois S,Stevanović S,Manoury B,Ramirez BC,Moris A

PA5-34754 was used in Western Blot to show that the calnexin cytosolic tail is required for this interaction.

Tue Dec 06 00:00:00 EST 2022

Prevalence and mechanisms of evolutionary contingency in human influenza H3N2 neuraminidase.

Nature communications

Lei R,Tan TJC,Hernandez Garcia A,Wang Y,Diefenbacher M,Teo C,Gopan G,Tavakoli Dargani Z,Teo QW,Graham CS,Brooke CB,Nair SK,Wu NC

PA5-34754 was used in Immunocytochemistry to show that among 70 natural mutations that are present in the NA of a recent human H3N2 strain, >10% are deleterious for an ancestral strain.

Fri Oct 28 00:00:00 EDT 2022

An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens.

eLife

Coukos R,Yao D,Sanchez MI,Strand ET,Olive ME,Udeshi ND,Weissman JS,Carr SA,Bassik MC,Ting AY

PA5-34754 was used in Immunocytochemistry-immunofluorescence to demonstrate a distinct regulatory role for EMC10 in the ER membrane complex, opposing the transmembrane-domain insertion activity of the complex.

Fri Aug 20 00:00:00 EDT 2021

Immunosuppression by Mutated Calreticulin Released from Malignant Cells.

Molecular cell

Liu P,Zhao L,Loos F,Marty C,Xie W,Martins I,Lachkar S,Qu B,Waeckel-Énée E,Plo I,Vainchenker W,Perez F,Rodriguez D,López-Otin C,van Endert P,Zitvogel L,Kepp O,Kroemer G

PA5-34754 was used in Western Blot to demonstrate the paracrine immunosuppressive effects for exon-9-mutated C-terminally modified calreticulin.

Thu Feb 20 00:00:00 EST 2020

Striatal infusion of cholesterol promotes dose-dependent behavioral benefits and exerts disease-modifying effects in Huntington's disease mice.

EMBO molecular medicine

Birolini G,Valenza M,Di Paolo E,Vezzoli E,Talpo F,Maniezzi C,Caccia C,Leoni V,Taroni F,Bocchi VD,Conforti P,Sogne E,Petricca L,Cariulo C,Verani M,Caricasole A,Falqui A,Biella G,Cattaneo E

PA5-34754 was used in Immunohistochemistry to indicate that cholesterol infusion to the striatum can exert a dose-dependent, disease-modifying effect and may be therapeutically relevant in HD.

Wed Oct 07 00:00:00 EDT 2020

Striatal infusion of cholesterol promotes dose-dependent behavioral benefits and exerts disease-modifying effects in Huntington's disease mice.

EMBO molecular medicine

Birolini G,Valenza M,Di Paolo E,Vezzoli E,Talpo F,Maniezzi C,Caccia C,Leoni V,Taroni F,Bocchi VD,Conforti P,Sogne E,Petricca L,Cariulo C,Verani M,Caricasole A,Falqui A,Biella G,Cattaneo E

PA5-34754 was used in Immunohistochemistry to indicate that cholesterol infusion to the striatum can exert a dose-dependent, disease-modifying effect and may be therapeutically relevant in HD.

Wed Oct 07 00:00:00 EDT 2020

Striatal infusion of cholesterol promotes dose-dependent behavioral benefits and exerts disease-modifying effects in Huntington's disease mice.

EMBO molecular medicine

Birolini G,Valenza M,Di Paolo E,Vezzoli E,Talpo F,Maniezzi C,Caccia C,Leoni V,Taroni F,Bocchi VD,Conforti P,Sogne E,Petricca L,Cariulo C,Verani M,Caricasole A,Falqui A,Biella G,Cattaneo E

PA5-34754 was used in Immunohistochemistry to indicate that cholesterol infusion to the striatum can exert a dose-dependent, disease-modifying effect and may be therapeutically relevant in HD.

Wed Oct 07 00:00:00 EDT 2020

Immunohistochemical staining reveals differential expression of ACSL3 and ACSL4 in hepatocellular carcinoma and hepatic gastrointestinal metastases.

Bioscience reports

Ndiaye H,Liu JY,Hall A,Minogue S,Morgan MY,Waugh MG

PA5-34754 was used in Western Blotting to conclude that analysis of ACSL3 and ACSL4 expression can distinguish different classes of hepatic tumours.

Thu Apr 30 00:00:00 EDT 2020

Immunohistochemical staining reveals differential expression of ACSL3 and ACSL4 in hepatocellular carcinoma and hepatic gastrointestinal metastases.

Bioscience reports

Ndiaye H,Liu JY,Hall A,Minogue S,Morgan MY,Waugh MG

PA5-34754 was used in Western Blotting to conclude that analysis of ACSL3 and ACSL4 expression can distinguish different classes of hepatic tumours.

Thu Apr 30 00:00:00 EDT 2020

Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.

The Journal of biological chemistry

Young PA,Senkal CE,Suchanek AL,Grevengoed TJ,Lin DD,Zhao L,Crunk AE,Klett EL,Füllekrug J,Obeid LM,Coleman RA

PA5-34754 was used in Immunocytochemistry to provide fundamental and detailed insights into protein interaction networks that control fatty acid metabolism.

Fri Oct 26 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Calnexin; FLJ26570; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; p90

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Gene Aliases: 1110069N15Rik; AI988026; CANX; CNX; D11Ertd153e; IP90; P90

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UniProt ID: (Human) P27824, (Rat) P35565, (Mouse) P35564

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Entrez Gene ID: (Sheep) 101122273, (Human) 821, (Rat) 29144, (Mouse) 12330

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Function(s)

glycoprotein binding calcium ion binding protein binding carbohydrate binding apolipoprotein binding ionotropic glutamate receptor binding poly(A) RNA binding unfolded protein binding metal ion binding chaperone

Process(es)

antigen processing and presentation of peptide antigen via MHC class I protein folding aging protein secretion antigen processing and presentation of exogenous peptide antigen via MHC class II protein folding in endoplasmic reticulum synaptic vesicle endocytosis chaperone-mediated protein folding clathrin-mediated endocytosis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Calnexin Polyclonal Antibody

Calnexin Antibody (PA5-34754) in ICC/IF

Product Details

PA5-34754

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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