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Calnexin Antibody (PA5-77839) in IHC (PFA)
Immunohistochemistry analysis of Calnexin in mouse back skin. Sample was fixed using Bouins fixative solution, incubated with Calnexin polyclonal antibody (Product # PA5-77839) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.
Product Details
PA5-77839
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Avian,
Bovine,
Dog,
Chicken,
Chimpanzee,
Fruit fly,
Guinea pig,
Hamster,
Human,
Mouse,
Sheep,
Pig,
Rabbit,
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735568
Product Specific Information
A 1:2,000 dilution of PA5-77839 was sufficient for detection of Calnexin in 10 µg of HeLa cell lysate by ECL immunoblot analysis.|Detects the C-terminal domain of Calnexin approximately 90kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.
Target Information
Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calmegin; Calnexin; FLJ26570; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; p90; pp90
Gene Aliases: 1110069N15Rik; AI988026; CANX; canx-a; canx-b; clgn; clnx; CNX; D11Ertd153e; IP90; P90; PP90
UniProt ID: (Dog) P24643, (Human) P27824, (Rat) P35565, (Mouse) P35564
synaptic vesicle endocytosis
clathrin-mediated endocytosis
antigen processing and presentation of peptide antigen via MHC class I
protein folding
aging
protein secretion
antigen processing and presentation of exogenous peptide antigen via MHC class II
protein folding in endoplasmic reticulum
chaperone-mediated protein folding
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