Proteintech

Calnexin Monoclonal Antibody (2A2C6)

Calnexin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

Calnexin Antibody (66903-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66903-1-Ig (Calnexin antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: staining with CoraLite555-Phalloidin.

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Product Details

66903-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000

View 25 publications 25 publications

Immunocytochemistry (ICC/IF)

1:400

View 10 publications 10 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2A2C6

Immunogen

Calnexin Fusion Protein Ag25535

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Calnexin; FLJ26570; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; p90

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Gene Aliases: 1110069N15Rik; AI988026; CANX; CNX; D11Ertd153e; IP90; P90

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UniProt ID: (Human) P27824, (Rat) P35565, (Mouse) P35564

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Entrez Gene ID: (Human) 821, (Rat) 29144, (Mouse) 12330

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Function(s)

glycoprotein binding calcium ion binding protein binding carbohydrate binding apolipoprotein binding ionotropic glutamate receptor binding poly(A) RNA binding unfolded protein binding metal ion binding chaperone

Process(es)

antigen processing and presentation of peptide antigen via MHC class I protein folding aging protein secretion antigen processing and presentation of exogenous peptide antigen via MHC class II protein folding in endoplasmic reticulum synaptic vesicle endocytosis chaperone-mediated protein folding clathrin-mediated endocytosis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Calnexin Monoclonal Antibody (2A2C6)

Calnexin Antibody (66903-1-IG) in ICC/IF

Product Details

66903-1-IG

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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