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Invitrogen
Calnexin Recombinant Rabbit Monoclonal Antibody (ARC0648)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
Calnexin Antibody (MA5-35588) in ICC/IF
Immunofluorescence analysis of CANX was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Calnexin Recombinant Rabbit Monoclonal Antibody (ARC0648) (Product # MA5-35588) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa... View More
Product Details
MA5-35588
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0648
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 493-592 of human Calnexin (P27824)
Conjugate
Form
Liquid
Concentration
0.55 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849488
Product Specific Information
Immunogen sequence: LPVFLVILFC CSGKKQTSGM EYKKTDAPQP DVKEEEEEKE EEKDKGDEEE EGEEKLEEKQ KSDAEEDGGT VSQEEEDRKP KAEEDEILNR SPRNRKPRRE
Target Information
Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calnexin; FLJ26570; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; p90
Gene Aliases: CANX; CNX; IP90; P90
UniProt ID: (Human) P27824
Entrez Gene ID: (Human) 821
antigen processing and presentation of peptide antigen via MHC class I
protein folding
aging
protein secretion
antigen processing and presentation of exogenous peptide antigen via MHC class II
protein folding in endoplasmic reticulum
synaptic vesicle endocytosis
chaperone-mediated protein folding
clathrin-mediated endocytosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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