Proteintech

Calreticulin Polyclonal Antibody

Calreticulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

Calreticulin Antibody (27298-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calreticulin antibody (Product # 27298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Calreticulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Calreticulin Antibody in Western Blot (WB)

Calreticulin Antibody in Flow Cytometry (Flow)

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Product Details

27298-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 17 publications 17 publications

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Calreticulin Fusion Protein Ag26064 (248-417 aa encoded by BC002500)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KKPEDWDEE MDGEWEPPVI QNPEYKGEWK PRQIDNPDYK GTWIHPEIDN PEYSPDPSIY AYDNFGVLGL DLWQVKSGTI FDNFLITNDE AYAEEFGNET WGVTKAAEKQ MKDKQDEEQR LKEEEEDKKR KEEEEAEDKE DDEDKDEDEE DEEDKEEDEE EDVPGQAKDE L (248-417 aa encoded by BC002500)

Target Information

Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Autoantigen RO; CABP3; CALBP; Calcium-binding protein 3; Calregulin; Calreticulin; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin)

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Gene Aliases: CALR; Calregulin; cC1qR; CRT; CRTC; HEL-S-99n; RO; SSA

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UniProt ID: (Human) P27797, (Mouse) P14211, (Rat) P18418

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Entrez Gene ID: (Human) 811, (Mouse) 12317, (Rat) 64202

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Function(s)

complement component C1q binding glycoprotein binding DNA binding mRNA binding integrin binding iron ion binding calcium ion binding protein binding zinc ion binding carbohydrate binding ubiquitin protein ligase binding peptide binding hormone binding protein binding involved in protein folding poly(A) RNA binding androgen receptor binding unfolded protein binding chaperone binding metal ion binding chaperone

Process(es)

negative regulation of transcription from RNA polymerase II promoter antigen processing and presentation of peptide antigen via MHC class I peptide antigen assembly with MHC class I protein complex regulation of transcription, DNA-templated protein folding protein export from nucleus cellular calcium ion homeostasis receptor-mediated endocytosis cell cycle arrest spermatogenesis positive regulation of cell proliferation positive regulation of gene expression negative regulation of translation protein maturation by protein folding cortical actin cytoskeleton organization response to estradiol negative regulation of intracellular steroid hormone receptor signaling pathway response to testosterone protein localization to nucleus protein folding in endoplasmic reticulum ATF6-mediated unfolded protein response regulation of meiotic nuclear division response to drug glucocorticoid receptor signaling pathway regulation of apoptotic process negative regulation of neuron differentiation positive regulation of DNA replication positive regulation of cell cycle negative regulation of transcription, DNA-templated negative regulation of retinoic acid receptor signaling pathway positive regulation of phagocytosis protein stabilization sequestering of calcium ion cardiac muscle cell differentiation chaperone-mediated protein folding cellular response to lithium ion cellular senescence positive regulation of substrate adhesion-dependent cell spreading positive regulation of dendritic cell chemotaxis response to organic substance cellular response to organic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Calreticulin Polyclonal Antibody

Calreticulin Antibody (27298-1-AP) in ICC/IF

Product Details

27298-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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