Invitrogen

Calreticulin Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

9 Published Figures

21 References

View all (47) Calreticulin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Calreticulin Antibody in Western Blot (WB)

FIGURE: 1 / 14

Calreticulin Antibody (PA1-902A) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of A-549 (Lane 1), HEK 293 (Lane 2) and Mouse Brain (Lane 3). The blot was probed with Anti-Calreticulin Chicken Polyclonal Antibody (Product # PA1-902A, 1:500-1:2000 dilution) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate (Product # A16054, 0.4 µg/mL, 1:2500 dilution). A 48 kDa band corresponding to Calreticulin was observed across the cell lines and tissue tested. Known quantity of protein samples were ... View More

Calreticulin Antibody in Immunocytochemistry, Fluorescence Resonance Energy Transfer (ICC/IF, FRET)

Calreticulin Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

Calreticulin Antibody in Immunocytochemistry (ICC/IF)

Calreticulin Antibody in Western Blot, Immunohistochemistry (Frozen) (WB, IHC (F))

Calreticulin Antibody in Western Blot, Flow Cytometry, Immunoprecipitation (WB, Flow, IP)

Calreticulin Antibody in Flow Cytometry (Flow)

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Product Details

PA1-902A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 5 publications 5 publications

Flow Cytometry (Flow)

View 3 publications 3 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Fluorescence Resonance Energy Transfer (FRET)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: K(24) E Q F L D G D A W T N R W V E S K H K(43).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2069607

Product Specific Information

PA1-902A detects calreticulin from canine, hamster, human, mouse and rat tissues.

PA1-902A has been successfully used in Western blot, immunocytochemistry and immunofluorescene procedures. By Western blot, this antibody detects an ~60-66 kDa protein representing calreticulin.

PA1-902A immunizing peptide corresponds to amino acid residues 24-43 from mouse calreticulin. PA1-902A immunizing peptide (Cat. # PEP-018) is available for use in neutralization and control experiments.

Target Information

Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A transgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis.

Transgenic research

Svarcbahs R,Blossom SM,Baffoe-Bonnie HS,Trychta KA,Greer LK,Pickel J,Henderson MJ,Harvey BK

PA1-902A was used in Immunohistochemistry (Frozen) to create a transgenic mouse model, LSL-SERCaMP, with a Gaussia luciferase (GLuc)-based, secreted ER calcium-modulated protein (SERCaMP), allowing the monitoring of cell-type specific exodosis in response to ER calcium dysregulation in various organs and tissues.

Thu Jun 01 00:00:00 EDT 2023

Comparative study of PRPH2 D2 loop mutants reveals divergent disease mechanism in rods and cones.

Cellular and molecular life sciences : CMLS

Ikelle L,Makia M,Lewis T,Crane R,Kakakhel M,Conley SM,Birtley JR,Arshavsky VY,Al-Ubaidi MR,Naash MI

PA1-902A was used in Immunohistochemistry to evaluate the role of three D2 loop cysteine mutants (Y141C, C213Y and C150S), alone or in combination.

Wed Jul 19 00:00:00 EDT 2023

Adaptive changes of telocytes in the urinary bladder of patients affected by neurogenic detrusor overactivity.

Journal of cellular and molecular medicine

Traini C,Fausssone-Pellegrini MS,Guasti D,Del Popolo G,Frizzi J,Serni S,Vannucchi MG

PA1-902A was used in Immunohistochemistry to show that neurogenic detrusor overactivity causes significant changes of all the telocytes.

Mon Jan 01 00:00:00 EST 2018

Calreticulin Release at an Early Stage of Death Modulates the Clearance by Macrophages of Apoptotic Cells.

Frontiers in immunology

Osman R,Tacnet-Delorme P,Kleman JP,Millet A,Frachet P

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Calreticulin Release at an Early Stage of Death Modulates the Clearance by Macrophages of Apoptotic Cells.

Frontiers in immunology

Osman R,Tacnet-Delorme P,Kleman JP,Millet A,Frachet P

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Calreticulin Release at an Early Stage of Death Modulates the Clearance by Macrophages of Apoptotic Cells.

Frontiers in immunology

Osman R,Tacnet-Delorme P,Kleman JP,Millet A,Frachet P

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease.

Nature communications

Lüningschrör P,Binotti B,Dombert B,Heimann P,Perez-Lara A,Slotta C,Thau-Habermann N,R von Collenberg C,Karl F,Damme M,Horowitz A,Maystadt I,Füchtbauer A,Füchtbauer EM,Jablonka S,Blum R,Üçeyler N,Petri S,Kaltschmidt B,Jahn R,Kaltschmidt C,Sendtner M

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Western Blot

Mon Oct 30 00:00:00 EDT 2017

The C-Terminal Acidic Region of Calreticulin Mediates Phosphatidylserine Binding and Apoptotic Cell Phagocytosis.

Journal of immunology (Baltimore, Md. : 1950)

Wijeyesakere SJ,Bedi SK,Huynh D,Raghavan M

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

The C-Terminal Acidic Region of Calreticulin Mediates Phosphatidylserine Binding and Apoptotic Cell Phagocytosis.

Journal of immunology (Baltimore, Md. : 1950)

Wijeyesakere SJ,Bedi SK,Huynh D,Raghavan M

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Concurrent MEK and autophagy inhibition is required to restore cell death associated danger-signalling in Vemurafenib-resistant melanoma cells.

Biochemical pharmacology

Martin S,Dudek-Perić AM,Maes H,Garg AD,Gabrysiak M,Demirsoy S,Swinnen JV,Agostinis P

PA1-902A was used in blocking or activating experiment to study the role of autophagy in melanoma

Sun Feb 01 00:00:00 EST 2015

Relative contribution of c1q and apoptotic cell-surface calreticulin to macrophage phagocytosis.

Journal of innate immunity

Verneret M,Tacnet-Delorme P,Osman R,Awad R,Grichine A,Kleman JP,Frachet P

PA1-902A was used in immunocytochemistry to study the role in macrophage phagocytosis of the interaction between c1q and calreticulin

Mon May 11 00:00:00 EDT 2015

Telocytes subtypes in human urinary bladder.

Journal of cellular and molecular medicine

Vannucchi MG,Traini C,Guasti D,Del Popolo G,Faussone-Pellegrini MS

PA1-902A was used in immunohistochemistry - paraffin section to describe human urinary bladder by phenotypically by transmission electron microscopy (TEM) and immunohistochemistry

Wed Oct 01 00:00:00 EDT 2014

Inner and outer portions of colonic circular muscle: ultrastructural and immunohistochemical changes in rat chronically treated with otilonium bromide.

PloS one

Traini C,Faussone-Pellegrini MS,Evangelista S,Mazzaferro K,Cipriani G,Santicioli P,Vannucchi MG

PA1-902A was used in immunohistochemistry - frozen section and western blot to study the ultrastructural and immunohistochemical changes of inner and outer portions of colonic circular muscle in rats treated with otilonium bromide chronically

Fri Apr 17 00:00:00 EDT 2015

Inner and outer portions of colonic circular muscle: ultrastructural and immunohistochemical changes in rat chronically treated with otilonium bromide.

PloS one

Traini C,Faussone-Pellegrini MS,Evangelista S,Mazzaferro K,Cipriani G,Santicioli P,Vannucchi MG

Fri Apr 17 00:00:00 EDT 2015

Endoplasmic reticulum calcium depletion impacts chaperone secretion, innate immunity, and phagocytic uptake of cells.

Journal of immunology (Baltimore, Md. : 1950)

Peters LR,Raghavan M

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Flow Cytometry

Fri Jul 15 00:00:00 EDT 2011

Endoplasmic reticulum calcium depletion impacts chaperone secretion, innate immunity, and phagocytic uptake of cells.

Journal of immunology (Baltimore, Md. : 1950)

Peters LR,Raghavan M

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Flow Cytometry

Fri Jul 15 00:00:00 EDT 2011

Endoplasmic reticulum calcium depletion impacts chaperone secretion, innate immunity, and phagocytic uptake of cells.

Journal of immunology (Baltimore, Md. : 1950)

Peters LR,Raghavan M

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Flow Cytometry

Fri Jul 15 00:00:00 EDT 2011

Endoplasmic reticulum calcium depletion impacts chaperone secretion, innate immunity, and phagocytic uptake of cells.

Journal of immunology (Baltimore, Md. : 1950)

Peters LR,Raghavan M

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Flow Cytometry

Fri Jul 15 00:00:00 EDT 2011

Endoplasmic reticulum calcium depletion impacts chaperone secretion, innate immunity, and phagocytic uptake of cells.

Journal of immunology (Baltimore, Md. : 1950)

Peters LR,Raghavan M

Published figure using Calreticulin polyclonal antibody (Product # PA1-902A) in Flow Cytometry

Fri Jul 15 00:00:00 EDT 2011

Chronic ethanol consumption in mice alters hepatocyte lipid droplet properties.

Alcoholism, clinical and experimental research

Orlicky DJ,Roede JR,Bales E,Greenwood C,Greenberg A,Petersen D,McManaman JL

PA1-902A was used in immunohistochemistry to study the effects of chronic ethanol consumption on hepatocyte lipid droplets in mice

Wed Jun 01 00:00:00 EDT 2011

The WFS1 gene, responsible for low frequency sensorineural hearing loss and Wolfram syndrome, is expressed in a variety of inner ear cells.

Histochemistry and cell biology

Cryns K,Thys S,Van Laer L,Oka Y,Pfister M,Van Nassauw L,Smith RJ,Timmermans JP,Van Camp G

PA1-902A was used in immunohistochemistry to study the expression of wolframin in mouse inner ear at different developmental stages

Sat Mar 01 00:00:00 EST 2003

Cytoplasmic domain of herpes simplex virus gE causes accumulation in the trans-Golgi network, a site of virus envelopment and sorting of virions to cell junctions.

Journal of virology

McMillan TN,Johnson DC

PA1-902A was used in immunocytochemistry to investigate the role of the CT domain of HSV gE in the trans-Golgi network.

Thu Feb 01 00:00:00 EST 2001

Bioinformatics

Protein Aliases: Autoantigen RO; CABP3; CALBP; Calcium-binding protein 3; Calregulin; Calreticulin; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin)

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Gene Aliases: CALR; Calregulin; cC1qR; CRP55; CRT; CRTC; ERp60; HACBP; HEL-S-99n; I79_008346; RO; SSA

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UniProt ID: (Human) P27797, (Chinese hamster) Q8K3H7, (Mouse) P14211, (Rat) P18418

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Entrez Gene ID: (Human) 811, (Dog) 476694, (Chinese hamster) 100689096, (Mouse) 12317, (Rat) 64202

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Function(s)

complement component C1q binding glycoprotein binding DNA binding mRNA binding integrin binding iron ion binding calcium ion binding protein binding zinc ion binding carbohydrate binding ubiquitin protein ligase binding peptide binding hormone binding protein binding involved in protein folding poly(A) RNA binding androgen receptor binding unfolded protein binding chaperone binding metal ion binding chaperone

Process(es)

negative regulation of transcription from RNA polymerase II promoter antigen processing and presentation of peptide antigen via MHC class I peptide antigen assembly with MHC class I protein complex regulation of transcription, DNA-templated protein folding protein export from nucleus cellular calcium ion homeostasis receptor-mediated endocytosis cell cycle arrest spermatogenesis positive regulation of cell proliferation positive regulation of gene expression negative regulation of translation protein maturation by protein folding cortical actin cytoskeleton organization response to estradiol negative regulation of intracellular steroid hormone receptor signaling pathway response to testosterone protein localization to nucleus protein folding in endoplasmic reticulum ATF6-mediated unfolded protein response regulation of meiotic nuclear division response to drug glucocorticoid receptor signaling pathway regulation of apoptotic process negative regulation of neuron differentiation positive regulation of DNA replication positive regulation of cell cycle negative regulation of transcription, DNA-templated negative regulation of retinoic acid receptor signaling pathway positive regulation of phagocytosis protein stabilization sequestering of calcium ion cardiac muscle cell differentiation chaperone-mediated protein folding cellular response to lithium ion cellular senescence positive regulation of substrate adhesion-dependent cell spreading positive regulation of dendritic cell chemotaxis response to organic substance cellular response to organic substance

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Calreticulin Polyclonal Antibody

Calreticulin Antibody (PA1-902A) in WB

Product Details

PA1-902A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Fluorescence Resonance Energy Transfer (FRET)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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