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Invitrogen
Calretinin Monoclonal Antibody (SP13)
FIGURE: 1 / 2
Calretinin Antibody (MA5-16328) in IHC (P)
Immunohistochemical (Paraffin) analysis of Calretinin using anti-Calretinin Monoclonal Antibody (Product # MA5-16328) in human mesothelioma at a dilution of 1:100. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated for 15 mins at room temperature and detected using an HRP conjugated c... View More
Product Details
MA5-16328
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP13
Immunogen
Recombinant full-length mouse calretinin protein
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Purification
Protein A
Storage buffer
PBS, pH 7.20, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2537847
Product Specific Information
This antibody is predicted to react with bovine, chicken and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is mesothelioma.
Target Information
Calretinin is encoded by the CALB2 gene located on chromosome 16. It is a troponin C superfamily protein having six EF-hand structural motifs. The EF motif allows the protein to bind calcium, and function in calcium signaling, calcium buffering, and modulating neuronal excitability. Calretinin is expressed in the mesothelium, mast cells, some neural cells, hair follicles, and fat cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 29 kDa calbindin; 29kD calretinin; 29kDa calbindin; calbindin 2, (29kD, calretinin); calbindin D29K; Calretinin; CR; testicular secretory protein Li 8
Gene Aliases: CAB29; CAL2; CALB2; CR
UniProt ID: (Human) P22676, (Rat) P47728, (Mouse) Q08331
Entrez Gene ID: (Human) 794, (Rat) 117059, (Mouse) 12308
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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