Invitrogen

Cannabinoid Receptor 1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

29 References

View all (25) Cannabinoid Receptor 1 antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Cannabinoid Receptor 1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

Cannabinoid Receptor 1 Antibody (PA1-743) in ICC/IF

Immunlocalization of rat CB1 transfected into AtT20 cells using Product # PA1-743.

Cannabinoid Receptor 1 Antibody in Immunohistochemistry (IHC)

Cannabinoid Receptor 1 Antibody in Western Blot (WB)

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Product Details

PA1-743

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:1,000

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Rat

Published species

Ferret, Horse, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A fusion protein containing the first 99 amino acid residues from human CB1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2245118

Product Specific Information

PA1-743 detects cannabinoid receptor 1 (CB1) from human and rat tissues as well as transfected rat CB1.

PA1-743 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~52 kDa protein representing CB1 from rat brain homogenate. Immunocytochemical staining of CB1 in AtT20 cells transfected with the rat CB1 gene with PA1-743 yields a pattern consistent with plasma membrane staining.

PA1-743 antigen is a fusion protein containing the first 99 amino acid residues of human CB1.

Target Information

This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

2-Arachidonoylglycerol Modulates CXCL12-Mediated Chemotaxis in Mantle Cell Lymphoma and Chronic Lymphocytic Leukemia.

Cancers

Merrien M,Wasik AM,Melén CM,Morsy MHA,Sonnevi K,Junlén HR,Christensson B,Wahlin BE,Sander B

PA1-743 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to suggest that 2-AG has a previously unrecognized role in the mobilization of lymphoma cells by effecting the CXCL12-induced migration and the CXCR4 signaling pathways, however, with different effects in MCL compared to CLL.

Fri Mar 03 00:00:00 EST 2023

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin.

Journal of veterinary internal medicine

Kupczyk P,Rykala M,Serek P,Pawlak A,Slowikowski B,Holysz M,Chodaczek G,Madej JP,Ziolkowski P,Niedzwiedz A

PA1-743 was used in Immunohistochemistry to represent a starting point for developing and translating new veterinary medicine-based pharmacotherapies using ECS as a possible target.

Fri Jul 01 00:00:00 EDT 2022

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin.

Journal of veterinary internal medicine

Kupczyk P,Rykala M,Serek P,Pawlak A,Slowikowski B,Holysz M,Chodaczek G,Madej JP,Ziolkowski P,Niedzwiedz A

PA1-743 was used in Immunohistochemistry to represent a starting point for developing and translating new veterinary medicine-based pharmacotherapies using ECS as a possible target.

Fri Jul 01 00:00:00 EDT 2022

Low-Dose Administration of Cannabigerol Attenuates Inflammation and Fibrosis Associated with Methionine/Choline Deficient Diet-Induced NASH Model via Modulation of Cannabinoid Receptor.

Nutrients

Aljobaily N,Krutsinger K,Viereckl MJ,Joly R,Menlove B,Cone B,Suppes A,Han Y

PA1-743 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to show that the low dose of CBG alleviated hepatic fibrosis and inflammation in MCD-induced NASH, however, the high dose of CBG treatment showed enhanced liver damage when compared to MCD only group.

Fri Dec 30 00:00:00 EST 2022

Mechanism of Diuresis and Natriuresis by Cannabinoids: Evidence for Inhibition of Na⁺-K⁺-ATPase in Mouse Kidney Thick Ascending Limb Tubules.

The Journal of pharmacology and experimental therapeutics

Ritter JK,Ahmad A,Mummalaneni S,Daneva Z,Dempsey SK,Li N,Li PL,Lyall V

PA1-743 was used in Immunohistochemistry to suggest that the mechanism of the cannabinoids involves inhibition of the sodium pump, Na+-K+-ATPase, in thick ascending limb cells and, likely, other proximal and distal tubular segments of the kidney nephron.

Fri Jan 01 00:00:00 EST 2021

Synthetic Cannabinoid Agonist WIN 55212-2 Targets Proliferation, Angiogenesis, and Apoptosis via MAPK/AKT Signaling in Human Endometriotic Cell Lines and a Murine Model of Endometriosis.

Frontiers in reproductive health

Lingegowda H,Miller JE,Marks RM,Symons LK,Alward T,Lomax AE,Koti M,Tayade C

Published figure using Cannabinoid Receptor 1 polyclonal antibody (Product # PA1-743) in Immunohistochemistry

Sat Oct 29 00:00:00 EDT 2022

A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells.

Lab on a chip

Coles BLK,Labib M,Poudineh M,Innes BT,Belair-Hickey J,Gomis S,Wang Z,Bader GD,Sargent EH,Kelley SO,van der Kooy D

PA1-743 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry(Paraffin), Western Blot to identify transcription factors (TFs) that play functional roles in maintenance, quiescence, and proliferation of RSCs.

Tue Nov 09 00:00:00 EST 2021

Modulation of Neurolipid Signaling and Specific Lipid Species in the Triple Transgenic Mouse Model of Alzheimer's Disease.

International journal of molecular sciences

González de San Román E,Llorente-Ovejero A,Martínez-Gardeazabal J,Moreno-Rodríguez M,Giménez-Llort L,Manuel I,Rodríguez-Puertas R

PA1-743 was used in Immunohistochemistry to test the involvement of the neurolipid-based signaling by endocannabinoids, lysophosphatidic acid, and sphingosine 1-phosphate in relation to the lipid deregulation seen in Alzheimer's disease.

Fri Nov 12 00:00:00 EST 2021

Modulation of Neurolipid Signaling and Specific Lipid Species in the Triple Transgenic Mouse Model of Alzheimer's Disease.

International journal of molecular sciences

González de San Román E,Llorente-Ovejero A,Martínez-Gardeazabal J,Moreno-Rodríguez M,Giménez-Llort L,Manuel I,Rodríguez-Puertas R

Fri Nov 12 00:00:00 EST 2021

Upregulation of CB₂ receptors in reactive astrocytes in canine degenerative myelopathy, a disease model of amyotrophic lateral sclerosis.

Disease models & mechanisms

Fernández-Trapero M,Espejo-Porras F,Rodríguez-Cueto C,Coates JR,Pérez-Díaz C,de Lago E,Fernández-Ruiz J

Published figure using Cannabinoid Receptor 1 polyclonal antibody (Product # PA1-743) in Immunohistochemistry

Mon May 01 00:00:00 EDT 2017

Neonatal Treatment with a Pegylated Leptin Antagonist Induces Sexually Dimorphic Effects on Neurones and Glial Cells, and on Markers of Synaptic Plasticity in the Developing Rat Hippocampal Formation.

Journal of neuroendocrinology

López-Gallardo M,Antón-Fernández A,Llorente R,Mela V,Llorente-Berzal A,Prada C,Viveros MP

PA1-743 was used in immunohistochemistry to study the effect of the neonatal leptin surge on hippocampal formation

Sat Aug 01 00:00:00 EDT 2015

Overactive cannabinoid 1 receptor in podocytes drives type 2 diabetic nephropathy.

Proceedings of the National Academy of Sciences of the United States of America

Jourdan T,Szanda G,Rosenberg AZ,Tam J,Earley BJ,Godlewski G,Cinar R,Liu Z,Liu J,Ju C,Pacher P,Kunos G

Published figure using Cannabinoid Receptor 1 polyclonal antibody (Product # PA1-743) in Immunofluorescence

Tue Dec 16 00:00:00 EST 2014

Cannabinoid CB1 receptor is expressed in chromophobe renal cell carcinoma and renal oncocytoma.

Clinical biochemistry

Larrinaga G,Sanz B,Blanco L,Perez I,Candenas ML,Pinto FM,Irazusta A,Gil J,López JI

PA1-743 was used in immunohistochemistry to study the expression of CB1 and CB2 cannabinoid receptor mRNA and protein in chromophobe renal cell carcinoma and renal oncocytoma

Wed May 01 00:00:00 EDT 2013

A Role for the Cannabinoid 1 Receptor in Neuronal Differentiation of Adult Spinal Cord Progenitors in vitro is Revealed through Pharmacological Inhibition and Genetic Deletion.

Frontiers in neuroscience

Sideris A,Bekker T,Chan WS,Montoya-Gacharna JV,Blanck TJ,Recio-Pinto E

PA1-743 was used in immunocytochemistry to use knock out technology and pharmacological inhibition to elucidate the role of the cannabinoid-1 receptor in the neuronal differentiation of adult spinal cord progenitors

Tue Oct 02 00:00:00 EDT 2012

Genetic and pharmacologic inactivation of cannabinoid CB1 receptor inhibits angiogenesis.

Blood

Pisanti S,Picardi P,Prota L,Proto MC,Laezza C,McGuire PG,Morbidelli L,Gazzerro P,Ziche M,Das A,Bifulco M

PA1-743 was used in western blot to investigate the importance of CB1 receptor in angiogenesis

Thu May 19 00:00:00 EDT 2011

Spinal cord injuries induce changes in CB1 cannabinoid receptor and C-C chemokine expression in brain areas underlying circuitry of chronic pain conditions.

Journal of neurotrauma

Knerlich-Lukoschus F,Noack M,von der Ropp-Brenner B,Lucius R,Mehdorn HM,Held-Feindt J

PA1-743 was used in immunohistochemistry to study the effect of spinal cord injuries on cannabinoid receptor and chemokine expression in brain areas involved in pain perception

Fri Apr 01 00:00:00 EDT 2011

Effect of capacitation on the endocannabinoid system of mouse sperm.

Molecular and cellular endocrinology

Catanzaro G,Battista N,Rossi G,Di Tommaso M,Pucci M,Pirazzi V,Cecconi S,Maccarrone M

PA1-743 was used in western blot to investigate the effect of capacitation on the spermic endocannabinoid system in mice

Mon Aug 22 00:00:00 EDT 2011

Effects of chronic bhang (cannabis) administration on the reproductive system of male mice.

Birth defects research. Part B, Developmental and reproductive toxicology

Banerjee A,Singh A,Srivastava P,Turner H,Krishna A

PA1-743 was used in western blot to investigate the effect of chronic bhang uptake on male reproductive physiology in mice

Wed Jun 01 00:00:00 EDT 2011

Levels of endocannabinoids and palmitoylethanolamide and their pharmacological manipulation in chronic granulomatous inflammation in rats.

Pharmacological research

De Filippis D,D'Amico A,Cipriano M,Petrosino S,Orlando P,Di Marzo V,Iuvone T

PA1-743 was used in western blot to investigate the influence of endocannabinoids and palmitoylethanolamide on chronic granulomatous inflammation

Thu Apr 01 00:00:00 EDT 2010

Cannabinoid CB₁ receptor is downregulated in clear cell renal cell carcinoma.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Larrinaga G,Sanz B,Pérez I,Blanco L,Cándenas ML,Pinto FM,Gil J,López JI

PA1-743 was used in immunohistochemistry and western blot to investigate the changes of cannabinoid receptors in clear cell renal cell carcinoma

Wed Dec 01 00:00:00 EST 2010

Cannabinoid CB₁ receptor is downregulated in clear cell renal cell carcinoma.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Larrinaga G,Sanz B,Pérez I,Blanco L,Cándenas ML,Pinto FM,Gil J,López JI

PA1-743 was used in immunohistochemistry and western blot to investigate the changes of cannabinoid receptors in clear cell renal cell carcinoma

Wed Dec 01 00:00:00 EST 2010

CB1 and CB2 cannabinoid receptor expression during development and in epileptogenic developmental pathologies.

Neuroscience

Zurolo E,Iyer AM,Spliet WG,Van Rijen PC,Troost D,Gorter JA,Aronica E

PA1-743 was used in immunohistochemistry to study the role of the CB1 and CB2 cannabinoid receptors in epileptogenic developmental pathologies

Wed Sep 29 00:00:00 EDT 2010

Involvement of the endocannabinoid system in periodontal healing.

Biochemical and biophysical research communications

Kozono S,Matsuyama T,Biwasa KK,Kawahara K,Nakajima Y,Yoshimoto T,Yonamine Y,Kadomatsu H,Tancharoen S,Hashiguchi T,Noguchi K,Maruyama I

PA1-743 was used in immunohistochemistry to study the role of endocannabinoid receptors in periodontal healing

Fri Apr 16 00:00:00 EDT 2010

Regulation of subthalamic neuron activity by endocannabinoids.

Synapse (New York, N.Y.)

Morera-Herreras T,Ruiz-Ortega JA,Taupignon A,Baufreton J,Manuel I,Rodriguez-Puertas R,Ugedo L

PA1-743 was used in immunohistochemistry to study the role of endocannabinoids in the regulation of subthalamic neuron activity

Wed Sep 01 00:00:00 EDT 2010

Activated endocannabinoid system in coronary artery disease and antiinflammatory effects of cannabinoid 1 receptor blockade on macrophages.

Circulation

Sugamura K,Sugiyama S,Nozaki T,Matsuzawa Y,Izumiya Y,Miyata K,Nakayama M,Kaikita K,Obata T,Takeya M,Ogawa H

PA1-743 was used in immunohistochemistry to study the possible role of the endocannabinoid system in patients with CAD and CB2 receptor blockade's antiinflammatory effect on human macrophages

Tue Jan 06 00:00:00 EST 2009

Opposing control of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis: in vitro and in vivo evidence.

The Journal of pharmacology and experimental therapeutics

Esposito G,Iuvone T,Savani C,Scuderi C,De Filippis D,Papa M,Di Marzo V,Steardo L

PA1-743 was used in western blot to investigate the effect of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis

Sat Sep 01 00:00:00 EDT 2007

Cannabinoid receptors in human astroglial tumors.

Journal of neurochemistry

Held-Feindt J,Dörner L,Sahan G,Mehdorn HM,Mentlein R

Published figure using Cannabinoid Receptor 1 polyclonal antibody (Product # PA1-743) in Immunofluorescence

Tue Aug 01 00:00:00 EDT 2006

Cannabinoid CB2 receptors and fatty acid amide hydrolase are selectively overexpressed in neuritic plaque-associated glia in Alzheimer's disease brains.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Benito C,Núñez E,Tolón RM,Carrier EJ,Rábano A,Hillard CJ,Romero J

PA1-743 was used in immunohistochemistry to study the expression of cannabinoid CB2 receptors and fatty acid amide hydrolase in neuritic plaque-associated glia in Alzheimer disease brains.

Wed Dec 03 00:00:00 EST 2003

Delta9-tetrahydrocannabinol selectively acts on CB1 receptors in specific regions of dorsal vagal complex to inhibit emesis in ferrets.

American journal of physiology. Gastrointestinal and liver physiology

Van Sickle MD,Oland LD,Mackie K,Davison JS,Sharkey KA

PA1-743 was used in immunohistochemistry to investigate the antiemetic effect of THC in the ferret

Mon Sep 01 00:00:00 EDT 2003

Cannabinoids acting on CB1 receptors decrease contractile performance in human atrial muscle.

Journal of cardiovascular pharmacology

Bonz A,Laser M,Küllmer S,Kniesch S,Babin-Ebell J,Popp V,Ertl G,Wagner JA

PA1-743 was used in immunohistochemistry to investigate the impact of cannabinoids on human atrial heart muscle

Tue Apr 01 00:00:00 EST 2003

Bioinformatics

Protein Aliases: Brain-type cannabinoid receptor; CANN6; Cannabinoid receptor 1; cannabinoid receptor 1 (brain); CB-R; CB1; CB1 receptor; central cannabinoid receptor; HGNC:2159; OTTHUMP00000016839; RP1-23D17.1

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Gene Aliases: CANN6; CB-R; CB1; CB1A; CB1K5; CB1R; CNR; CNR1; Skr6; SKR6R

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UniProt ID: (Human) P21554, (Rat) P20272

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Entrez Gene ID: (Human) 1268, (Rat) 25248

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Function(s)

cannabinoid receptor activity drug binding protein binding G-protein coupled receptor

Process(es)

positive regulation of acute inflammatory response to antigenic stimulus G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger adenylate cyclase-modulating G-protein coupled receptor signaling pathway spermatogenesis axonal fasciculation aging response to nutrient memory positive regulation of neuron projection development sensory perception of pain positive regulation of fever generation negative regulation of fatty acid beta-oxidation regulation of synaptic transmission, GABAergic response to lipopolysaccharide negative regulation of mast cell activation negative regulation of dopamine secretion response to nicotine cannabinoid signaling pathway response to cocaine glucose homeostasis positive regulation of apoptotic process negative regulation of ion transport response to morphine response to ethanol negative regulation of action potential negative regulation of blood pressure positive regulation of blood pressure regulation of insulin secretion negative regulation of nitric-oxide synthase activity regulation of synaptic transmission, glutamatergic maternal process involved in female pregnancy regulation of feeding behavior regulation of penile erection G-protein coupled receptor signaling pathway learning or memory regulation of lipid metabolic process

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Cannabinoid Receptor 1 Polyclonal Antibody

Cannabinoid Receptor 1 Antibody (PA1-743) in ICC/IF

Product Details

PA1-743

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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