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Rockland
Carboxypeptidase B Polyclonal Antibody, Biotin
FIGURE: 1 / 1
Carboxypeptidase B Antibody (200-406-049-0100) in WB
Western Blot of Rabbit anti-Carboxypeptidase B antibody Biotin conjugated. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carboxypeptidase B antibody Biotin conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP conjugated rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking buffer for Fluorescent Western Blotting (MB-070) at RT for 30 min. Predicted/Observed size: ~35 kDa
Product Details
200-406-049-0100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Pig
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carboxypeptidase B [Porcine Pancreas]
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-CARBOXYPEPTIDASE B (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas]. Cross reactivity against Carboxypeptidase B from other sources may occur but have not been specifically determined.
Target Information
Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Carboxypeptidase B; carboxypeptidase B1 (tissue); procarboxypeptidase B
Gene Aliases: CPB; CPB1
UniProt ID: (Pig) P09955
Entrez Gene ID: (Pig) 397341
Performance Guarantee
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