Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Cardiac Troponin C Recombinant Rabbit Monoclonal Antibody (8Z4V9)
FIGURE: 1 / 1
Cardiac Troponin C Antibody (MA5-42838) in WB
Western blot analysis of Cardiac Troponin C in extracts of various cell lines. Samples were incubated with Cardiac Troponin C Monoclonal antibody (Product # MA5-42838) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-42838
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8Z4V9
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TNNC1 (P63316)
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911979
Product Specific Information
Positive test controls include: Mouse heart, Rat heart .
Immunogen sequence: MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GKSEEELSDL
Target Information
Troponin (Tn) is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments, and as a result of calcium-ion uptake by troponin-C, the elements of the muscle thin filament undergo a series of allosteric changes which allow the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cardiac 3; CMD1FF; CMD2A; CMH7; MGC116817; RCM1; TN-C; Tn1; Tni; TNN I3; TNNC 1; TNNI3; troponin C, cardiac/slow skeletal; Troponin C, slow skeletal and cardiac muscles; Troponin I cardiac; TroponinI
Gene Aliases: AI874626; cTnC; cTnI; TnC; Tncc; Tnnc1
UniProt ID: (Mouse) P19123
Entrez Gene ID: (Rat) 290561, (Mouse) 21924
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support