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FIGURE: 1 / 1
Cas9 Antibody (PA5-90171) in WB
Western blot analysis of Cas9 in extracts of 293T cells and 293T transfected with CAS9 protein. Samples were incubated with Cas9 Polyclonal antibody (Product # PA5-90171) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
Product Details
PA5-90171
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 450-730 of streptococcus pyogenes m1 gas CAS9 (NP_269215.1).
Conjugate
Form
Liquid
Concentration
1.37 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805947
Product Specific Information
Positive Samples: HeLa
Immunogen sequence: YYVGPLARGN SRFAWMTRKS EETITPWNFE EVVDKGASAQ SFIERMTNFD KNLPNEKVLP KHSLLYEYFT VYNELTKVKY VTEGMRKPAF LSGEQKKAIV DLLFKTNRKV TVKQLKEDYF KKIECFDSVE ISGVEDRFNA SLGTYHDLLK IIKDKDFLDN EENEDILEDI VLTLTLFEDR EMIEERLKTY AHLFDDKVMK QLKRRRYTGW GRLSRKLING IRDKQSGKTI LDFLKSDGFA NRNFMQLIHD DSLTFKEDIQ KAQVSGQGDS LHEHIANLAG S
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APAF-3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP 9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase-9; Caspase-9 precursor; CASPASE-9c; Caspase9; CRISPR-Cas9; CRISPR9; CTD; ICE-LAP6; ICE-like apoptotic protease 6; OTTHUMP00000002324; protein phosphatase 1, regulatory subunit 56; RNCASP9; RP11-265F14.3; s aureus
Gene Aliases: APAF-3; APAF3; CASP9; ICE-LAP6; MCH6; PPP1R56
UniProt ID: (Human) P55211
Entrez Gene ID: (Human) 842
proteolysis
apoptotic process
cellular response to DNA damage stimulus
aging
intrinsic apoptotic signaling pathway in response to DNA damage
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
platelet formation
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
cellular response to UV
signal transduction in response to DNA damage
positive regulation of neuron apoptotic process
response to antibiotic
cellular response to dexamethasone stimulus
extrinsic apoptotic signaling pathway in absence of ligand
regulation of response to DNA damage stimulus
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