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Invitrogen
Caspase 1 (cleaved Asp297) Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
Caspase 1 (cleaved Asp297) Antibody (PA5-77886) in WB
Knockout of Caspase 1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR917229_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Caspase 1 was performed by loading 30 µg of HT29 Cas9 (Lane 1), HT29 Cas9 treated with 0.1 µg/mL of LPS for 4hrs followed by 1mM of ATP for 2hrs (Lane 2), HT29 CASP1 KO (Lane 3) and HT29 C... View More
Product Details
PA5-77886
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human Caspase 1 (cleaved Asp297). The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2735573
Product Specific Information
Positive Control: conditioned medium of treated THP-1, THP-1 conditioned medium (500 ng/mL LPS treatment for 4 hr in serum free and 1 mM ATP treatment for 1.5 hr)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Caspase 1 (cleaved Asp297) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 1 (cleaved Asp297) was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. Caspase 1 (cleaved Asp297) has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CASP-1; CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase-1; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
Gene Aliases: CASP1; ICE; IL1BC; IL1BCE; P45
UniProt ID: (Human) P29466
Entrez Gene ID: (Human) 834
response to hypoxia
proteolysis
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
signal transduction
regulation of autophagy
protein processing
response to lipopolysaccharide
interleukin-1 beta production
response to ATP
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of interleukin-1 alpha secretion
positive regulation of interleukin-1 beta secretion
regulation of inflammatory response
mitochondrial depolarization
membrane hyperpolarization
pyroptosis
cellular response to mechanical stimulus
cellular response to organic substance
programmed necrotic cell death
toxin transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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