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Caspase 10 Antibody (PA5-120032) in WB
Western blot analysis of Caspase 10 in extracts of Jurkat cells. Samples were incubated with Caspase 10 Polyclonal antibody (Product # PA5-120032) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Jurkat cells were treated by Etoposide (25 µM) at 37°C for 5 hours. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-120032
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 220-521 of human Caspase-10 (NP_1167592)
Conjugate
Form
Liquid
Concentration
1.38 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913604
Product Specific Information
Positive test controls include: Jurkat, NIH/3T3.
Immunogen sequence: VKTFLEALPQ ESWQNKHAGS NGNRATNGAP SLVSRGMQGA SANTLNSETS TKRAAVYRMN RNHRGLCVIV NNHSFTSLKD RQGTHKDAEI LSHVFQWLGF TVHIHNNVTK VEMEMVLQKQ KCNPAHADGD CFVFCILTHG RFGAVYSSDE ALIPIREIMS HFTALQCPRL AEKPKLFFIQ ACQGEEIQPS VSIEADALNP EQAPTSLQDS IPAEADFLLG LATVPGYVSF RHVEEGSWYI QSLCNHLKKL VPRHEDILSI LTAVNDDVSR RVDKQGTKKQ MPQPAFTLRK KLVFPVPLDA LS
Target Information
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apoptotic protease Mch-4; CASP-10; caspase 10 apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; Caspase-10; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2
Gene Aliases: ALPS2; CASP10; FLICE2; MCH4
UniProt ID: (Human) Q92851
Entrez Gene ID: (Human) 843
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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